CALORIMETRIC STUDY OF THE INFLUENCE OF HYDRATION AND pH UPON THE THERMALLY-INDUCED CONFORMATIONAL TRANSITION OF PROTEINASE K
文献类型：期刊论文
| 作者 | Wang Bangning; Tan Fu |
| 刊名 | ACTA PHYSICO-CHIMICA SINICA
 |
| 出版日期 | 1991 |
| 卷号 | 7期号:1页码:43-48 |
| 关键词 | Proteinase k Thermal Danaturation Dsc (Differential Scanning Calorimetry) Hydration Ph |
| ISSN号 | 1000-6818 |
| DOI | 10.3866/PKU.WHXB19910107 |
| 英文摘要 | Differential scanning calorimetry was used to investigate the thermal denaturation of the proteinase K E2 3, 4, 21, 14, in the temperature range from 310 to 450K. The influence of hydration and pH upon the thermal denaturation was studied in the range of total water content from 0.048 to 4.39g H2O/g dry enzyme and in the pH range from 3.20 to 8.49, respectively, The dependence of the denaturational temperature T-d, the specific enthalpy of denaturation Delta H-d vial the maximum of excess apparent specific heat C-sx(max) upon the degree of hydration h and the pH of the buffer is presented respectively. The relationship between the denaturational. temperature To and the water content f(B) of the Flory-Garrett's type Besides this, the thermodynamic characteristics of the thermal denaturation of proteinase K are different from those reported of g globular proteins, doable stranded coiled-coils and triple-3tranded coiled coils,The influence of primary hydration water upon the thermodynamic parameters of the thermal denaturation is the greatest, The external variable, the pH of the buffer used to dissolve the enzyme influences its thermostability to some extent. |
| 语种 | 英语 |
| WOS记录号 | WOS:000207573300007 |
| 出版者 | PEKING UNIV PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/54979]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang Bangning |
| 作者单位 | Acad Sinica, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Bangning,Tan Fu. CALORIMETRIC STUDY OF THE INFLUENCE OF HYDRATION AND pH UPON THE THERMALLY-INDUCED CONFORMATIONAL TRANSITION OF PROTEINASE K[J]. ACTA PHYSICO-CHIMICA SINICA,1991,7(1):43-48. |
| APA | Wang Bangning,&Tan Fu.(1991).CALORIMETRIC STUDY OF THE INFLUENCE OF HYDRATION AND pH UPON THE THERMALLY-INDUCED CONFORMATIONAL TRANSITION OF PROTEINASE K.ACTA PHYSICO-CHIMICA SINICA,7(1),43-48. |
| MLA | Wang Bangning,et al."CALORIMETRIC STUDY OF THE INFLUENCE OF HYDRATION AND pH UPON THE THERMALLY-INDUCED CONFORMATIONAL TRANSITION OF PROTEINASE K".ACTA PHYSICO-CHIMICA SINICA 7.1(1991):43-48. |
入库方式： OAI收割
来源：化学研究所
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