Investigation on the precipitation, microenvironment and recovery of protein in CO2-expanded reverse micellar solution
文献类型：期刊论文
| 作者 | Chen, J; Zhang, JL; Liu, DX; Liu, ZM; Han, BX; Yang, GY |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2004 |
| 卷号 | 33期号:1页码:33-37 |
| 关键词 | Compressed Co2 Transmission Electron Microscopy Reverse Micelles |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2003.08.011 |
| 英文摘要 | The effect of compressed CO2 on the properties of protein (trypsin) in the reverse micelles of sodium bis(2-ethylhexyl)sulfosuccinate AOT/decane/water has been studied. UV-vis spectrum was used to determine the precipitation of trypsin in the reverse micelles, which reveals that trypsin can be precipitated from the reverse micelles by compressed CO2 at suitable pressures, while the surfactant AOT remains in the solution. FT-IR spectroscopy was used to investigate the microenvironment of trypsin in the CO2-expanded reverse micelles, which shows that the micropolarity inside the micelle cores is changed gradually with the CO2 pressure. Using compressed CO2 as antisolvent, the protein in the reverse micelles can be recovered, and trypsin nanoparticles with the size of less than 15 nm were obtained, which was characterized by transmission electron microscopy (TEM). (C) 2003 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000188512400005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/55503]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Han, BX |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Grp 111, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, J,Zhang, JL,Liu, DX,et al. Investigation on the precipitation, microenvironment and recovery of protein in CO2-expanded reverse micellar solution[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2004,33(1):33-37. |
| APA | Chen, J,Zhang, JL,Liu, DX,Liu, ZM,Han, BX,&Yang, GY.(2004).Investigation on the precipitation, microenvironment and recovery of protein in CO2-expanded reverse micellar solution.COLLOIDS AND SURFACES B-BIOINTERFACES,33(1),33-37. |
| MLA | Chen, J,et al."Investigation on the precipitation, microenvironment and recovery of protein in CO2-expanded reverse micellar solution".COLLOIDS AND SURFACES B-BIOINTERFACES 33.1(2004):33-37. |
入库方式： OAI收割
来源：化学研究所
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