Mass spectrometric evidence of heparin disaccharides for the catalytic characterization of a novel endolytic heparinase
文献类型：期刊论文
| 作者 | Chao, YP; Xiong, SX; Cheng, XL; Qian, SJ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004-12-01 |
| 卷号 | 36期号:12页码:840-844 |
| 关键词 | Electrospray Ionization-mass Spectrometry Heparin Disaccharides Heparinase High Performance Liquid Chromatography Sphingobacterium Sp. |
| ISSN号 | 1672-9145 |
| 英文摘要 | Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight hepatitis with different characteristics. Porcine intestinal mucosa heparin was degraded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccharide components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak Ill represented an N-acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate-substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin. |
| 语种 | 英语 |
| WOS记录号 | WOS:000225986000009 |
| 出版者 | SHANGHAI INST BIOCHEMISTRY, ACADEMIA SINICA |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/55543]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Qian, SJ |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mass Spect, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chao, YP,Xiong, SX,Cheng, XL,et al. Mass spectrometric evidence of heparin disaccharides for the catalytic characterization of a novel endolytic heparinase[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(12):840-844. |
| APA | Chao, YP,Xiong, SX,Cheng, XL,&Qian, SJ.(2004).Mass spectrometric evidence of heparin disaccharides for the catalytic characterization of a novel endolytic heparinase.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(12),840-844. |
| MLA | Chao, YP,et al."Mass spectrometric evidence of heparin disaccharides for the catalytic characterization of a novel endolytic heparinase".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.12(2004):840-844. |
入库方式： OAI收割
来源：化学研究所
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