Quartz crystal biosensor for real-time kinetic analysis of interaction between human TNF-alpha and monoclonal antibodies
文献类型：期刊论文
| 作者 | Liu, Y; Zhang, W; Yu, X; Zhang, HW; Zhao, R; Shangguan, D; Li, Y; Shen, BF; Liu, GQ |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2004-05-01 |
| 卷号 | 99期号:2-3页码:416-424 |
| 关键词 | Quartz Crystal Biosensor Kinetic Analysis Human Tnf-alpha Genetic Algorithm Elisa |
| ISSN号 | 0925-4005 |
| DOI | 10.1016/j.snb.2003.12.010 |
| 英文摘要 | Quartz crystal microbalance (QCM) biosensor integrated in a flow injection analysis (FIA) system was used for the real-time investigation of the interaction between human TNF-alpha (hTNF-alpha) and its monoclonal antibodies (mAbs). Biologically active hTN-F-alpha trimer was immobilized on the gold electrodes of the piezoelectric crystals and the binding interactions of the two immobilized antigen with two anti-hTNF-alpha monoclonal antibodies (Z8 and Z12) were followed as changes in the resonant frequency of the modified crystals. The resultant sensorgrams were rapidly analyzed by using an in-house kinetic analysis software based on genetic algorithm (GA) to derive both the kinetic rate constants (k(ass) and k(diss)) and equilibrium dissociation constant (K-D) for antigen-antibody interactions. For the interactions, K-D were 2.31 x 10(-7) and 5.42 x 10(-7) M for Z8 and Z12, respectively. The generally good agreement found between biosensor data and the affinities known from conventional equilibrium enzyme-linked immunosorbent assay (ELISA) validates this analytical method. The study shows the potential usefulness of QCM biosensor technology for biomolecular interaction analysis. (C) 2004 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000221580100033 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/55741]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, GQ |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 2.Inst Basic Med Sci, Beijing 100850, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Y,Zhang, W,Yu, X,et al. Quartz crystal biosensor for real-time kinetic analysis of interaction between human TNF-alpha and monoclonal antibodies[J]. SENSORS AND ACTUATORS B-CHEMICAL,2004,99(2-3):416-424. |
| APA | Liu, Y.,Zhang, W.,Yu, X.,Zhang, HW.,Zhao, R.,...&Liu, GQ.(2004).Quartz crystal biosensor for real-time kinetic analysis of interaction between human TNF-alpha and monoclonal antibodies.SENSORS AND ACTUATORS B-CHEMICAL,99(2-3),416-424. |
| MLA | Liu, Y,et al."Quartz crystal biosensor for real-time kinetic analysis of interaction between human TNF-alpha and monoclonal antibodies".SENSORS AND ACTUATORS B-CHEMICAL 99.2-3(2004):416-424. |
入库方式： OAI收割
来源：化学研究所
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