Study of the effect of metal ion on the specific interaction between protein and aptamer by atomic force microscopy
文献类型：期刊论文
| 作者 | Jiang, YX; Wang, J; Fang, XH; Bai, CL |
| 刊名 | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
 |
| 出版日期 | 2004-07-01 |
| 卷号 | 4期号:6页码:611-615 |
| 关键词 | Afm Rupture Force Aptamer Immunoglobulin e (Ige) |
| ISSN号 | 1533-4880 |
| 英文摘要 | We have studied the effect of metal ions on the specific interaction between a protein, immunoglobulin E (IgE), and its 37-nt DNA aptamer with atomic force microscopy (AFM). Protein aptamers are a new class of synthetic single-stranded DNA/RNA oligonucleotide generated from in vitro selection to selectively bind with target proteins. The IgE aptamers have been developed and are expected to be promising reagents in IgE detection and new anti-allergic drug development. It is known that the presence of metal ions in the buffer usually has a strong effect on the affinity of single-stranded DNA for protein. In this work, the effect of two representative monovalent ion and divalent ion on the binding of IgE and the aptamer has been studied at the single-molecule level. The results from the AFM force measurements show that the metal ions not only reduce the single-molecular rupture force but also reduce the number of bonds formed between IgE and the aptamer. |
| 语种 | 英语 |
| WOS记录号 | WOS:000224698300011 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/55819]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, XH |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, YX,Wang, J,Fang, XH,et al. Study of the effect of metal ion on the specific interaction between protein and aptamer by atomic force microscopy[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2004,4(6):611-615. |
| APA | Jiang, YX,Wang, J,Fang, XH,&Bai, CL.(2004).Study of the effect of metal ion on the specific interaction between protein and aptamer by atomic force microscopy.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,4(6),611-615. |
| MLA | Jiang, YX,et al."Study of the effect of metal ion on the specific interaction between protein and aptamer by atomic force microscopy".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 4.6(2004):611-615. |
入库方式： OAI收割
来源：化学研究所
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