Protein target selection for structural genomics of thermoanaerobacter tengcongensis
文献类型：期刊论文
| 作者 | Pei, JF; Lai, LH |
| 刊名 | ACTA PHYSICO-CHIMICA SINICA
 |
| 出版日期 | 2005-05-01 |
| 卷号 | 21期号:5页码:499-503 |
| 关键词 | Thermoanaerobacter Tengcongensis Structural Genomics Target Selection Protein Structure Blast Psi-blast Protonet |
| ISSN号 | 1000-6818 |
| DOI | 10.3866/PKU.WHXB20050508 |
| 英文摘要 | 20 proteins. were selected from Thermoanaerobacter tengcongensis proteome as the pilot group of targets for a structural genomics project which aims to fish for proteins with new folds and important biological functions. BLASTP, PSI-BLAST and TBLASTN searches were performed against pdb, nr and est-human databases, respectively, in order to find proteins that are least homologous to PDB proteins and most homologous to human proteins. Transmembrane protein predicting programs were used to classify membrane and soluble proteins. The selection was then made with further considerations on functions and parameters such as molecular weight and cysteine ratio. A validation made by the ProtoNet protein sequence hierarchical classification database showed that 95% of our target proteins locate in clusters that have no structures solved. Secondary structures and shapes of the target proteins predicted by PredictProtein program provided useful information for the experimental efforts. 8 selected proteins were cloned, expressed and purified, of which 2 were crystallized. Our experimental results and some recently published results show that some of our selected proteins bear new fold. |
| 语种 | 英语 |
| WOS记录号 | WOS:000229305800008 |
| 出版者 | PEKING UNIV PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/56037]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lai, LH |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China 2.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, JF,Lai, LH. Protein target selection for structural genomics of thermoanaerobacter tengcongensis[J]. ACTA PHYSICO-CHIMICA SINICA,2005,21(5):499-503. |
| APA | Pei, JF,&Lai, LH.(2005).Protein target selection for structural genomics of thermoanaerobacter tengcongensis.ACTA PHYSICO-CHIMICA SINICA,21(5),499-503. |
| MLA | Pei, JF,et al."Protein target selection for structural genomics of thermoanaerobacter tengcongensis".ACTA PHYSICO-CHIMICA SINICA 21.5(2005):499-503. |
入库方式： OAI收割
来源：化学研究所
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