Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface
文献类型：期刊论文
| 作者 | Huang, ZX; Ji, DM; Xia, AD |
| 刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| 出版日期 | 2005-05-05 |
| 卷号 | 257-58页码:203-209 |
| 关键词 | Single Molecule Fluorescence Fluctuation Reverse Intersystem Crossing On/off-time Dynamic Processes |
| ISSN号 | 0927-7757 |
| DOI | 10.1016/j.colsurfa.2004.10.108 |
| 英文摘要 | Single molecule fluorescence spectroscopy is a powerful tool to reveal the fluorescence fluctuation dynamics of a fluorescence molecule immobilized on a surface. These fluctuations are correlated in time under different excitation intensities with different repetition rate, which may provide the information for us to describe the reasonable excited state processes as well as the surrounding environments. In this report, we showed the first results on the fluorescence fluctuations of a single Cy5 molecule at the air-glass interface excited with different conditions by using far-field confocal laser microscopy in conjunction with multichannel scale (MCS) and time-correlated single photon counting (TCSPC). We found that the on/off-time and count rates showed different behaviors under different excitation modes. It was found that the excitation with the high repetition rate and/or strong intensity may cause high count rate, and increase the possibility of reverse intersystem crossing from T,, to S. state. which may lead to longer on-time and shorter off-time. Furthermore, the photoinduced dynamic processes excited with strong peak power described by a five-level model under continuous wave (CW) and pulsed excitations with high repetition rate, it can be roughly simplified by a three-level scheme under pulse laser at lower repetition rate. (c) 2004 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000228321500039 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/56119]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xia, AD |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, ZX,Ji, DM,Xia, AD. Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2005,257-58:203-209. |
| APA | Huang, ZX,Ji, DM,&Xia, AD.(2005).Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,257-58,203-209. |
| MLA | Huang, ZX,et al."Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 257-58(2005):203-209. |
入库方式： OAI收割
来源：化学研究所
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