Chiral recognition of dextran sulfate with D- and L-cystine studied by multiwavelength surface plasmon resonance
文献类型：期刊论文
| 作者 | Chen, Y; Huang, HW; Yu, X; Qi, L |
| 刊名 | CARBOHYDRATE RESEARCH
 |
| 出版日期 | 2005-09-05 |
| 卷号 | 340期号:12页码:2024-2029 |
| 关键词 | Dextran Sulfate Cystine Chiral Recognition Surface Plasmon Resonance |
| ISSN号 | 0008-6215 |
| DOI | 10.1016/j.carres.2005.06.012 |
| 英文摘要 | A multiwavelength surface plasmon resonance (mwSPR) approach has been developed to study the chiral discrimination between D- and L-cystine (Cys). A monolayer of the two enantiomers was separately assembled on a pair of gold films of about 50 nm in thickness and their resonance wavelength shifts, Delta lambda, were measured under a continuous flow of an identical chiral probe solution. Dextran sulfate (DS) was found to be an excellent chiral probe because it has rich chiral centers and is large enough to produce sensitive mwSPR response. The chiral discrimination was investigated either by Delta lambda(max), the maximum resonance wavelength shift in recognition equilibrium, or by recognition kinetics (Delta lambda vs time). The equilibrium data showed that D-Cys yielded always the smaller Delta lambda(max) as compared to L-Cys at pH 5.0 or above. This differentiation was enlarged by raising the probe content and became naught at pH < 4.5. The kinetic results showed that, as pH increased from 5.0 to 7.5, the non-equilibrium Delta lambda for D-Cys rose above the level for L-Cys at the first 30 s of recognition but came back gradually to its equilibrium position after about 150 s, with crossing at 50-150 s depending on DS concentration. This phenomenon was thought to be the result of molecular orientation adjustment after DS binding to D-Cys. Both kinetic and thermodynamic mechanisms were thus considered to be deeply involved in the investigated chiral recognition system. (c) 2005 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000231189100012 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/56143]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Y |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Y,Huang, HW,Yu, X,et al. Chiral recognition of dextran sulfate with D- and L-cystine studied by multiwavelength surface plasmon resonance[J]. CARBOHYDRATE RESEARCH,2005,340(12):2024-2029. |
| APA | Chen, Y,Huang, HW,Yu, X,&Qi, L.(2005).Chiral recognition of dextran sulfate with D- and L-cystine studied by multiwavelength surface plasmon resonance.CARBOHYDRATE RESEARCH,340(12),2024-2029. |
| MLA | Chen, Y,et al."Chiral recognition of dextran sulfate with D- and L-cystine studied by multiwavelength surface plasmon resonance".CARBOHYDRATE RESEARCH 340.12(2005):2024-2029. |
入库方式： OAI收割
来源：化学研究所
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