Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109
文献类型：期刊论文
| 作者 | Nomura, CT; Taguchi, K; Gan, ZH; Kuwabara, K; Tanaka, T; Takase, K; Doi, Y |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 71期号:8页码:4297-4306 |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/AEM.71.8.4297-4306.2005 |
| 英文摘要 | Polyhydroxyalkanoates (PHAs) are biologically produced polyesters that have potential application as biodegradable plastics. Especially important are the short-chain-length-medium-chain-length (SCL-MCL) PHA copolymers, which have properties ranging from thermoplastic to elastomeric, depending on the ratio of SCL to MCL monomers incorporated into the copolymer. Because of the potential wide range of applications for SCL-MCL PIIA copolymers, it is important to develop and characterize metabolic pathways for SCL-MCL PHA production. In previous studies, coexpression of PHA synthase genes and the 3-ketoacyl-acyl carrier protein reductase gene (fabG) in recombinant Escherichia coli has been shown to enhance PHA production from related carbon sources such as fatty acids. In this study, a new fabG gene from Pseudomonas sp. 61-3 was cloned and its gene product characterized. Results indicate that the Pseudomonas sp. 61-3 and E. coli FabG proteins have different substrate specificities in vitro. The current study also presents the first evidence that coexpression of fabG genes from either E. coli or Pseudomonas sp. 61-3 with fabH(F87T) and PHA synthase genes can enhance the production of SCL-MCL PHA copolymers from nonrelated carbon sources. Differences in the substrate specificities of the FabG proteins were reflected in the monomer composition of the polymers produced by recombinant E. coli. SCL-MCL PHA copolymer isolated from a recombinant E. coli strain had improved physical properties compared to the SCL homopolymer poly-3-hydroxybutyrate. This study defines a pathway to produce SCL-MCL PHA copolymer from the fatty acid biosynthesis that may impact on PHA production in recombinant organisms. |
| 语种 | 英语 |
| WOS记录号 | WOS:000231165500020 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/56175]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Nomura, CT |
| 作者单位 | 1.Riken Inst Phys & Chem Res, Polymer Chem Lab, Wako, Saitama 3510198, Japan 2.Chinese Acad Sci, Inst Chem, CAS Key Lab Polymer Sci & Mat, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nomura, CT,Taguchi, K,Gan, ZH,et al. Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2005,71(8):4297-4306. |
| APA | Nomura, CT.,Taguchi, K.,Gan, ZH.,Kuwabara, K.,Tanaka, T.,...&Doi, Y.(2005).Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,71(8),4297-4306. |
| MLA | Nomura, CT,et al."Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 71.8(2005):4297-4306. |
入库方式： OAI收割
来源：化学研究所
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