Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species
文献类型：期刊论文
| 作者 | Shi, HL; Timmins, G; Monske, M; Burdick, A; Kalyanaraman, B; Liu, Y; Clement, JL; Burchiel, S; Liu, KJ |
| 刊名 | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2005-05-01 |
| 卷号 | 437期号:1页码:59-68 |
| 关键词 | Bmpo Dmpo Depmpo Deppepo Spin Trap Superoxide Radical Anion Epr Cell |
| ISSN号 | 0003-9861 |
| DOI | 10.1016/j.abb.2005.02.028 |
| 英文摘要 | Electron paramagnetic resonance with spin trapping is a useful technique to detect reactive oxygen species, such as superoxide radical anion (O-2(center dot-)), a key species in many biological processes. We evaluated the abilities of four spin traps in trapping cell-generated O-2(center dot-): 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide (BMPO), 2-diethoxyphosphoryl-2-phenethyl-3,4-dihydro-2H-pyrrole N-oxide (DEPPEPO), 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Optimal experimental conditions for obtaining maximal signal intensity of O-2(center dot-) adduct in a cellular system were first studied. The maximal intensities of BMPO, DEPMPO, and DMPO adducts were similar while DEPPEPO did not trap cell-generated O-2(center dot-) induced by 1,6-benzo[a]pyrene quinone in a human mammary epithelial cell line (MCF-10A). BMPO and DEPMPO adducts were more stable, considering the stability of their maximal signal, than DMPO adduct in the tested cellular systems. In addition, we observed that O-2(center dot-) spin adducts were reduced to their corresponding hydroxyl adducts in the cellular system. The selection of optimal spin trap in trapping cell-generated O-2(center dot-) is discussed. (c) 2005 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000228513900007 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/56223]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, KJ |
| 作者单位 | 1.Univ New Mexico, Coll Pharm, Albuquerque, NM 87131 USA 2.Med Coll Wisconsin, Biophys Res Inst, Milwaukee, WI 53226 USA 3.Med Coll Wisconsin, Free Rad Res Ctr, Milwaukee, WI 53226 USA 4.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 5.CNRS, UMR 6517, Lab SREP, F-13397 Marseille, France 6.Univ Aix Marseille 1, Case 521, F-13397 Marseille, France 7.Univ Aix Marseille 3, Case 521, F-13397 Marseille, France |
| 推荐引用方式 GB/T 7714 | Shi, HL,Timmins, G,Monske, M,et al. Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2005,437(1):59-68. |
| APA | Shi, HL.,Timmins, G.,Monske, M.,Burdick, A.,Kalyanaraman, B.,...&Liu, KJ.(2005).Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,437(1),59-68. |
| MLA | Shi, HL,et al."Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 437.1(2005):59-68. |
入库方式： OAI收割
来源：化学研究所
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