The interaction between severe acute respiratory syndrome coronavirus 3C-like proteinase and a dimeric inhibitor by capillary electrophoresis
文献类型：期刊论文
| 作者 | Ding, L; Zhang, XX; Wei, P; Fan, KQ; Lai, LH |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 343期号:1页码:159-165 |
| 关键词 | Binding Constants Capillary Electrophoresis Interface Inhibitor Sars 3c-like Proteinase Thermodynamic Parameters |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2005.04.027 |
| 英文摘要 | 3C-like proteinase of severe acute respiratory syndrome (SARS) coronavirus has been demonstrated to be a key target for drug design against SARS. The interaction between SARS coronavirus 3C-like (3CL) proteinase and an octapeptide interface inhibitor was studied by affinity capillary electrophoresis (ACE). The binding constants were estimated by the change of migration time of the analytes in the buffer solution containing different concentrations of SARS 3CL proteinase. The results showed that SARS 3CL proteinase was able to complex with the octapeptide competitively, with binding constants of 2.44 x 10(4) m-(1) at 20 degrees C and 2.11 x 10(4) M-1 at 37 degrees C. In addition, the thermodynamic parameters deduced reveal that hydrophobic interaction might play major roles, along with electrostatic force, in the binding process. The ACE method used here could be developed to be an effective and simple way of applying large-scale drug screening and evaluation. (c) 2005 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000230858600017 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/56617]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, XX |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Peking Univ, State Key Lab Struct Chem Unstable & Stable Speci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 3.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, L,Zhang, XX,Wei, P,et al. The interaction between severe acute respiratory syndrome coronavirus 3C-like proteinase and a dimeric inhibitor by capillary electrophoresis[J]. ANALYTICAL BIOCHEMISTRY,2005,343(1):159-165. |
| APA | Ding, L,Zhang, XX,Wei, P,Fan, KQ,&Lai, LH.(2005).The interaction between severe acute respiratory syndrome coronavirus 3C-like proteinase and a dimeric inhibitor by capillary electrophoresis.ANALYTICAL BIOCHEMISTRY,343(1),159-165. |
| MLA | Ding, L,et al."The interaction between severe acute respiratory syndrome coronavirus 3C-like proteinase and a dimeric inhibitor by capillary electrophoresis".ANALYTICAL BIOCHEMISTRY 343.1(2005):159-165. |
入库方式： OAI收割
来源：化学研究所
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