Hydrolysis reaction analysis of L-alpha-distearoylphosphatidyleholine monolayer catalyzed by phospholipase A(2) with polarization-modulated infrared reflection absorption spectroscopy
文献类型：期刊论文
| 作者 | Wang, XL; Zheng, SP; He, Q; Brezesinski, G; Mohwald, H; Li, JB |
| 刊名 | LANGMUIR
 |
| 出版日期 | 2005-02-01 |
| 卷号 | 21期号:3页码:1051-1054 |
| ISSN号 | 0743-7463 |
| DOI | 10.1021/la0492052 |
| 英文摘要 | The hydrolysis reaction of L-alpha-distearoylphosphatidylcholine (DSPC) monolayers catalyzed by phospholipase A(2) (PLA(2)) has been studied using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) with film balance measurements. The PM-IRRAS analysis provides quantitative information about the reaction efficiency at different surface pressures. It was found that the reaction efficiency of L-DSPC monolayer hydrolysis catalyzed by PLA(2) decreased with increasing surface pressure. At zero pressure (lift-off point), the hydrolysis reaction efficiency has the highest value of 45%. Increasing surface pressure leads to the decrease of the hydrolysis efficiency. Since the surface pressure is above 20 mN/m, the hydrolysis reaction nearly stopped. PM-IRRAS technique provides a powerful means to study the hydrolysis process catalyzed by phospholipase A(2) at the air/water interface. |
| 语种 | 英语 |
| WOS记录号 | WOS:000226614200039 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/57219]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, JB |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid & Interface Sci,Int Joint Lab, Beijing 100080, Peoples R China 2.Max Planck Inst Colloids & Interfaces, D-14476 Potsdam, Germany |
| 推荐引用方式 GB/T 7714 | Wang, XL,Zheng, SP,He, Q,et al. Hydrolysis reaction analysis of L-alpha-distearoylphosphatidyleholine monolayer catalyzed by phospholipase A(2) with polarization-modulated infrared reflection absorption spectroscopy[J]. LANGMUIR,2005,21(3):1051-1054. |
| APA | Wang, XL,Zheng, SP,He, Q,Brezesinski, G,Mohwald, H,&Li, JB.(2005).Hydrolysis reaction analysis of L-alpha-distearoylphosphatidyleholine monolayer catalyzed by phospholipase A(2) with polarization-modulated infrared reflection absorption spectroscopy.LANGMUIR,21(3),1051-1054. |
| MLA | Wang, XL,et al."Hydrolysis reaction analysis of L-alpha-distearoylphosphatidyleholine monolayer catalyzed by phospholipase A(2) with polarization-modulated infrared reflection absorption spectroscopy".LANGMUIR 21.3(2005):1051-1054. |
入库方式： OAI收割
来源：化学研究所
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