中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Hydrolysis reaction analysis of L-alpha-distearoylphosphatidyleholine monolayer catalyzed by phospholipase A(2) with polarization-modulated infrared reflection absorption spectroscopy

文献类型:期刊论文

作者Wang, XL; Zheng, SP; He, Q; Brezesinski, G; Mohwald, H; Li, JB
刊名LANGMUIR
出版日期2005-02-01
卷号21期号:3页码:1051-1054
ISSN号0743-7463
DOI10.1021/la0492052
英文摘要The hydrolysis reaction of L-alpha-distearoylphosphatidylcholine (DSPC) monolayers catalyzed by phospholipase A(2) (PLA(2)) has been studied using polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) with film balance measurements. The PM-IRRAS analysis provides quantitative information about the reaction efficiency at different surface pressures. It was found that the reaction efficiency of L-DSPC monolayer hydrolysis catalyzed by PLA(2) decreased with increasing surface pressure. At zero pressure (lift-off point), the hydrolysis reaction efficiency has the highest value of 45%. Increasing surface pressure leads to the decrease of the hydrolysis efficiency. Since the surface pressure is above 20 mN/m, the hydrolysis reaction nearly stopped. PM-IRRAS technique provides a powerful means to study the hydrolysis process catalyzed by phospholipase A(2) at the air/water interface.
语种英语
WOS记录号WOS:000226614200039
出版者AMER CHEMICAL SOC
源URL[http://ir.iccas.ac.cn/handle/121111/57219]  
专题中国科学院化学研究所
通讯作者Li, JB
作者单位1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid & Interface Sci,Int Joint Lab, Beijing 100080, Peoples R China
2.Max Planck Inst Colloids & Interfaces, D-14476 Potsdam, Germany
推荐引用方式
GB/T 7714
Wang, XL,Zheng, SP,He, Q,et al. Hydrolysis reaction analysis of L-alpha-distearoylphosphatidyleholine monolayer catalyzed by phospholipase A(2) with polarization-modulated infrared reflection absorption spectroscopy[J]. LANGMUIR,2005,21(3):1051-1054.
APA Wang, XL,Zheng, SP,He, Q,Brezesinski, G,Mohwald, H,&Li, JB.(2005).Hydrolysis reaction analysis of L-alpha-distearoylphosphatidyleholine monolayer catalyzed by phospholipase A(2) with polarization-modulated infrared reflection absorption spectroscopy.LANGMUIR,21(3),1051-1054.
MLA Wang, XL,et al."Hydrolysis reaction analysis of L-alpha-distearoylphosphatidyleholine monolayer catalyzed by phospholipase A(2) with polarization-modulated infrared reflection absorption spectroscopy".LANGMUIR 21.3(2005):1051-1054.

入库方式: OAI收割

来源:化学研究所

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