Detection of local polarity of alpha-lactalbumin by n-terminal specific labeling with a new tailor-made fluorescent probe
文献类型：期刊论文
| 作者 | Dong, SY; Ma, HM; Duan, XJ; Chen, XQ; Li, J |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2005 |
| 卷号 | 4期号:1页码:161-166 |
| 关键词 | Analytical Methods Local Polarity Detection Fluorescent Probe N-terminal Specific Labeling Alpha-lactalbumin |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/pr049814v |
| 英文摘要 | To detect the local polarity such as the N-terminal domain of a protein molecule, 3-(4-chloro-6-hydrazino-1,3,5-triazinylamino)-7-(dimethylamino)-2-methylphenazine has been designed and synthesized as a polarity-sensitive fluorescent probe by using an s-triazine ring as a backbone, neutral red and hydrazine as a polarity-sensitive fluorophore, and a labeling group, respectively. The fluorescence properties of the probe have been characterized. The probe has the following features: (1) stable in various solvents; (2) the long-wavelength emission of > 550 nm that can avoid the interferences of the background fluorescence shorter than 500 nm from common biomacromolecules; and (3) the maximum emission wavelength (Am) sensitive to solvent polarity only but not to pH and temperature. The hydrazino group in such a probe reacts readily with an active carbonyl produced by transamination of a protein molecule, leading to N-terminal specific attachment of the fluorophore and thereby allowing the monitoring of local polarity. With this probe, the polarity of the N-terminal domain in both native and heat-denatured a.-lactalbumin has been first determined, which corresponds to that with a dielectric constant of about 16, and the hydrophobic core near the N-terminus is found to be conservative for heating. The present strategy may provide a general method to study the local environmental changes of a protein molecule under different denaturation conditions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000227287000019 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/57583]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, HM |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, SY,Ma, HM,Duan, XJ,et al. Detection of local polarity of alpha-lactalbumin by n-terminal specific labeling with a new tailor-made fluorescent probe[J]. JOURNAL OF PROTEOME RESEARCH,2005,4(1):161-166. |
| APA | Dong, SY,Ma, HM,Duan, XJ,Chen, XQ,&Li, J.(2005).Detection of local polarity of alpha-lactalbumin by n-terminal specific labeling with a new tailor-made fluorescent probe.JOURNAL OF PROTEOME RESEARCH,4(1),161-166. |
| MLA | Dong, SY,et al."Detection of local polarity of alpha-lactalbumin by n-terminal specific labeling with a new tailor-made fluorescent probe".JOURNAL OF PROTEOME RESEARCH 4.1(2005):161-166. |
入库方式： OAI收割
来源：化学研究所
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