A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin III
文献类型：期刊论文
| 作者 | Zhao, R; Luo, H; Shangguan, D; Liu, GQ |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2005-02-25 |
| 卷号 | 816期号:1-2页码:175-181 |
| 关键词 | Viscose Fiber High Performance Affinity Chromatography Heparin Antithrombin Iii |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2004.11.030 |
| 英文摘要 | Viscose fiber, a regenerated cellulose, was evaluated for using as a novel matrix for high performance affinity chromatography. With a one-step activation with epichlorohydrin, heparin can be readily covalently attached to the matrix. This heparin-viscose fiber material was used for purifying antithrombin III (AT III) from human plasma. The purity of the AT III from this one-step purification is 93% as measured by SDS-PAGE and the protein recovery yield is about 90%. This column is highly specific as described by the dissociation constant of the complex of immobilized heparin and AT III, which was 2.83 x 10(-5) mol/L. And more important, this viscose fiber material demonstrated its excellent mechanical property that allows the flow rate to reach up to 900 cm/h or more. (C) 2004 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000226879800023 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/57865]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, R |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, R,Luo, H,Shangguan, D,et al. A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin III[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,816(1-2):175-181. |
| APA | Zhao, R,Luo, H,Shangguan, D,&Liu, GQ.(2005).A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin III.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,816(1-2),175-181. |
| MLA | Zhao, R,et al."A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin III".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 816.1-2(2005):175-181. |
入库方式： OAI收割
来源：化学研究所
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