In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy
文献类型：期刊论文
| 作者 | Ye Chang; Chen Chunying; Chen Zhen; Meng Huan; Xing Li; Jiang Yaxin; Yuan Hui; Xing Gengmei; Zhao Feng; Zhao Yuliang |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 51期号:9页码:1060-1064 |
| 关键词 | Fluorescence Microscopy Real Time Imaging Fullerene Derivative C-60(c(Cooh)(2))(2) Cross Cellular Membrane Fluorescence Labeling |
| ISSN号 | 1001-6538 |
| DOI | 10.1007/s11434-006-1060-1 |
| 英文摘要 | The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C-60 derivative C-60(C(COOH)(2))(2), a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C-60(C(COOH)(2))(2) can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C-60(C(COOH)(2))(2) can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 x 10(-2) to 1 x 10(2) mg/L, C-60(C(COOH)(2))(2) does not show any detectable cytotoxicity. |
| 语种 | 英语 |
| WOS记录号 | WOS:000238882500007 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/57961]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao Yuliang |
| 作者单位 | 1.Chinese Acad Sci, Lab Bioenvironm Hlth Sci Nanoscale Mat, Inst High Energy Phys, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China 3.Natl Ctr Nanosci & Technol, Beijing 100080, Peoples R China 4.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye Chang,Chen Chunying,Chen Zhen,et al. In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy[J]. CHINESE SCIENCE BULLETIN,2006,51(9):1060-1064. |
| APA | Ye Chang.,Chen Chunying.,Chen Zhen.,Meng Huan.,Xing Li.,...&Wei Taotao.(2006).In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy.CHINESE SCIENCE BULLETIN,51(9),1060-1064. |
| MLA | Ye Chang,et al."In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy".CHINESE SCIENCE BULLETIN 51.9(2006):1060-1064. |
入库方式： OAI收割
来源：化学研究所
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