Genetic cloning and functional expression in Escherichia coli of an archaerhodopsin gene from Halorubrum xinjiangense
文献类型：期刊论文
| 作者 | Feng, J; Liu, HC; Chu, JF; Zhou, PJ; Tang, JA; Liu, SJ |
| 刊名 | EXTREMOPHILES
 |
| 出版日期 | 2006-02-01 |
| 卷号 | 10期号:1页码:29-33 |
| 关键词 | Archaerhodopsin Bacteriorhodopsin Purple Membrane Halorubrum |
| ISSN号 | 1431-0651 |
| DOI | 10.1007/s00792-005-0468-x |
| 英文摘要 | Pairs of PCR primers that targeted the archae/ bacteriorhodopsin gene were used to clone the archaerhodopsin (aR) gene or Halorubrum xinjiangense strain BD-1(T), and this gene was sequenced and functionally expressed in Escherichia coli. Recombinant E. coli cells harboring the plasmid carrying this gene became slightly Purple or blue depending on whether they were supplemented with all-trans retinal or 3,4-dihydroretinal, respectively, during induction with IPTG. The purple and blue membranes from the recombinant E. coli showed maximal absorption at 555 and 588 nm respectively, which are different from maximal absorption at 568 nm of the wild-type purple membrane. Purple membranes from the recombinant E. coli and From strain BD-1(T) were investigated in parallel. The E. coli purple membrane was fabricated into films and photoelectric responses were observed that depended on the light-on and light-off stimuli. |
| 语种 | 英语 |
| WOS记录号 | WOS:000235607500003 |
| 出版者 | SPRINGER TOKYO |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/58139]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Feng, J |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, J,Liu, HC,Chu, JF,et al. Genetic cloning and functional expression in Escherichia coli of an archaerhodopsin gene from Halorubrum xinjiangense[J]. EXTREMOPHILES,2006,10(1):29-33. |
| APA | Feng, J,Liu, HC,Chu, JF,Zhou, PJ,Tang, JA,&Liu, SJ.(2006).Genetic cloning and functional expression in Escherichia coli of an archaerhodopsin gene from Halorubrum xinjiangense.EXTREMOPHILES,10(1),29-33. |
| MLA | Feng, J,et al."Genetic cloning and functional expression in Escherichia coli of an archaerhodopsin gene from Halorubrum xinjiangense".EXTREMOPHILES 10.1(2006):29-33. |
入库方式： OAI收割
来源：化学研究所
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