Determination of evodiamine and rutecarpine in human serum by liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Wen, DW; Li, CC; Liu, YP; Liao, YP; Liu, HW |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 385期号:6页码:1075-1081 |
| 关键词 | Evodiamine Rutecarpine Lc-ms Lc-ms/ms Serum |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-006-0493-3 |
| 英文摘要 | Evodiamine and rutecarpine are two kinds of indole alkaloids contained in the fruit of Evodiae fructus, which have been shown to exhibit various bioactivities in humans. A liquid chromatography-tandem mass spectrometric method (LC-MS/MS) was developed for the determination of evodiamine and rutecarpine in human serum. The serum was extracted by solid-phase extraction (SPE) and analyzed using a C18 column and a mobile phase consisting of methanol-water (85:15) solution containing 5 mmol/L ammonium formate at a flow rate of 0.5 mL/min. The mass spectrometer was operated in positive mode, employing the extracted ion chromatogram (EIC) for detection and quantitation of evodiamine (m/z 288) and rutecarpine (m/z 304). Good linear relationships between the peak area and the concentration were obtained in the ranges of 5.2-1040 ng/mL and 10.2-1020 ng/mL, with correlation coefficients (r) of 0.999 and 0.998, for evodiamine and rutecarpine, respectively. The repeatabilities (RSD, n=6) of quantitation for evodiamine and rutecarpine were 2.18-4.00% and 2.99-5.67%, respectively, and the recovery ranged from 90.5% to 98.1%. A comparative study of the different ionization and quantitation modes, including ESI-MS, ESI-MS/MS, APCI-MS and APCI-MS/MS, was also accomplished. The MS/MS fragmentation mechanism of the base peak ([M+H](+), m/z 304) of evodiamine was investigated in order to identify the analytes in more complicated body fluid samples. |
| 语种 | 英语 |
| WOS记录号 | WOS:000238706900012 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/58153]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, HW |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem,Inst Analyt Chem, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Mol Engn Minist Educ, Inst Analyt Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, DW,Li, CC,Liu, YP,et al. Determination of evodiamine and rutecarpine in human serum by liquid chromatography-tandem mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,385(6):1075-1081. |
| APA | Wen, DW,Li, CC,Liu, YP,Liao, YP,&Liu, HW.(2006).Determination of evodiamine and rutecarpine in human serum by liquid chromatography-tandem mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,385(6),1075-1081. |
| MLA | Wen, DW,et al."Determination of evodiamine and rutecarpine in human serum by liquid chromatography-tandem mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 385.6(2006):1075-1081. |
入库方式： OAI收割
来源：化学研究所
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