Spectroscopic studies of the interaction between hypocrellin B and human serum albumin
文献类型：期刊论文
| 作者 | Zhao, BZ; Song, LM; Liu, X; Xie, H; Zhao, JQ |
| 刊名 | BIOORGANIC & MEDICINAL CHEMISTRY
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 14期号:7页码:2428-2432 |
| 关键词 | Hypocrellins Human Serum Albumin Fluorescence Ligand Binding Site Ph-sensitive Fluorescence |
| ISSN号 | 0968-0896 |
| DOI | 10.1016/j.bmc.2005.11.022 |
| 英文摘要 | Previous work has proved that hypocrellin B (HB) binds to human serum albumin (HSA) at a specific site instead of distributed randomly on the surface of a protein. In the current work, further investigation by using bilirubin as a site I marker indicates that HB can compete for the same site with bilirubin, suggesting that the HB binding site is located at sub-domain IIA (site I) of HSA. Moreover, bound to HSA, the HB fluorescence was found to be pH sensitive in physiological range (pH 6.0-8.0). The increasing of binding constant of HB to HSA in the pH range 6-8 also indicates that the N <-> B transition modulates the micro-environment changes of the binding site and influences considerably the binding between HB and HSA. Furthermore, picosecond time-resolved fluorescence spectra of HB-HSA complex in PBS indicate an additional short-lived component compared to that for HB in benzene, which may be assigned to the process of electron transfer from Trp-214 to HB. (c) 2005 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000236015000028 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/58495]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, JQ |
| 作者单位 | Chinese Acad Sci, Key Lab Photochem, Ctr Mol Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, BZ,Song, LM,Liu, X,et al. Spectroscopic studies of the interaction between hypocrellin B and human serum albumin[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2006,14(7):2428-2432. |
| APA | Zhao, BZ,Song, LM,Liu, X,Xie, H,&Zhao, JQ.(2006).Spectroscopic studies of the interaction between hypocrellin B and human serum albumin.BIOORGANIC & MEDICINAL CHEMISTRY,14(7),2428-2432. |
| MLA | Zhao, BZ,et al."Spectroscopic studies of the interaction between hypocrellin B and human serum albumin".BIOORGANIC & MEDICINAL CHEMISTRY 14.7(2006):2428-2432. |
入库方式： OAI收割
来源：化学研究所
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