Fluorescence studies on the microenvironments of proteins in CO2-expanded reverse micellar solutions
文献类型：期刊论文
| 作者 | Chen, Jing; Zhang, Jianling; Wu, Yishi; Han, Buxing; Liu, Dongxia; Li, Zhonghao; Li, Junchun; Ai, Xicheng |
| 刊名 | JOURNAL OF SUPERCRITICAL FLUIDS
 |
| 出版日期 | 2006-08-01 |
| 卷号 | 38期号:1页码:103-110 |
| 关键词 | Fluorescence Studies Rnase a Cytochrome c |
| ISSN号 | 0896-8446 |
| DOI | 10.1016/j.supflu.2005.11.010 |
| 英文摘要 | The effect of compressed CO2 on the microenvironment of the two proteins (cytochrome c and RNase A) in sodium bis(2-ethylhexyl)sulfosuccinate (AOT) reverse micelles was investigated using high-pressure steady-state fluorescence and time-resolved fluorescence. It is found that RNase A can shift from the water core to the interface of the reverse micellar cores as CO2 is dissolved, while cytochrome c maintains at the interface of the micellar core. As the pressure reaches to a high enough value, the proteins can be precipitated from the reverse micelle, which has been detected by high-pressure UV-vis technique. The results show that cytochrome c was easier to be precipitated from the reverse micellar solution by CO2 than RNase A. The possible reasons for these behaviors were discussed based on the changes of the microenvironment of the two proteins, which could be tuned by the dissolution of compressed CO2. (C) 2005 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000240071100013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/58609]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Han, Buxing |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jing,Zhang, Jianling,Wu, Yishi,et al. Fluorescence studies on the microenvironments of proteins in CO2-expanded reverse micellar solutions[J]. JOURNAL OF SUPERCRITICAL FLUIDS,2006,38(1):103-110. |
| APA | Chen, Jing.,Zhang, Jianling.,Wu, Yishi.,Han, Buxing.,Liu, Dongxia.,...&Ai, Xicheng.(2006).Fluorescence studies on the microenvironments of proteins in CO2-expanded reverse micellar solutions.JOURNAL OF SUPERCRITICAL FLUIDS,38(1),103-110. |
| MLA | Chen, Jing,et al."Fluorescence studies on the microenvironments of proteins in CO2-expanded reverse micellar solutions".JOURNAL OF SUPERCRITICAL FLUIDS 38.1(2006):103-110. |
入库方式： OAI收割
来源：化学研究所
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