Use IgG-Eu-IDPA to detect antigen IOV by time resolved Fluoroimmunoassay
文献类型：期刊论文
| 作者 | Li, ZD; Lu, HT; Yue, JC; Fan, CH; Li, W; Luo, ZY |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2006-02-01 |
| 卷号 | 33期号:2页码:190-195 |
| 关键词 | Fluorescence Spectrum Fluorescence Probe Time Resolved Fluoroimmunoassay |
| ISSN号 | 1000-3282 |
| 英文摘要 | Lanthanide chelates have been successfully developed as fluorescence probes and widely used for highly sensitive time resolved fluoroimmunoassay. However, Lanthanide chelates are excited by UV-light which do a lot of damage to the biomolecules and cells. IgG-Eu-IDPA which can be excited by visible light (the maximum excite wavelength is 565nm) was compounded. The other spectra properties such as fluorescence lifetime, fluorescence intensity at different pH, fluorescence intensity in different concentration were measured. It was used as a fluorescence probe to detect antigen IOV with the self-built time resolved luminoscope. Its sensitivity is much higher than that of ordinary luminoscope. The results indicate that IgG-Eu-IDPA can be a new potential fluorescence probe for high sensitivity M situ and in vivo analysis. |
| 语种 | 英语 |
| WOS记录号 | WOS:000235488200013 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59145]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Luo, ZY |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Beijing Technol & Business Univ, Dept Biol, Beijing 100037, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 4.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, ZD,Lu, HT,Yue, JC,et al. Use IgG-Eu-IDPA to detect antigen IOV by time resolved Fluoroimmunoassay[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2006,33(2):190-195. |
| APA | Li, ZD,Lu, HT,Yue, JC,Fan, CH,Li, W,&Luo, ZY.(2006).Use IgG-Eu-IDPA to detect antigen IOV by time resolved Fluoroimmunoassay.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,33(2),190-195. |
| MLA | Li, ZD,et al."Use IgG-Eu-IDPA to detect antigen IOV by time resolved Fluoroimmunoassay".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 33.2(2006):190-195. |
入库方式： OAI收割
来源：化学研究所
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