Proteome analysis of up-regulated proteins in the rat spinal cord induced by transection injury
文献类型：期刊论文
| 作者 | Ding, QX; Wu, Z; Guo, YJ; Zhao, CJ; Jia, YF; Kong, FW; Chen, BY; Wang, HX; Xiong, SX; Que, HP |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2006 |
| 卷号 | 6期号:2页码:505-518 |
| 关键词 | Spinal Cord Injury Regeneration Up-regulation |
| ISSN号 | 1615-9853 |
| DOI | 10.1002/pmic.200500296 |
| 英文摘要 | The inability of the CNS to regenerate in adult mammals propels us to reveal associated proteins involved in the injured CNS. In this paper, either thoracic laminectomy (as sham control) or thoracic spinal cord transection was performed on male adult rats. Five days after surgery, the whole spinal cord tissue was dissected and fractionated into water-soluble (dissolved in Tris buffer) and water-insoluble (dissolved in a solution containing chaotropes and surfactants) portions for 2-DE. Protein identification was performed by MS and further confirmed by Western blot. As a result, over 30 protein spots in the injured spinal cord were shown to be up-regulated no less than 1.5-fold. These identified proteins possibly play various roles during the injury and repair process and may be functionally categorized as several different groups, such as stress-responsive and metabolic changes, lipid and protein degeneration, neural survival and regeneration. In particular, over-expression of 11-zinc finger protein and glypican may be responsible for the inhibition of axonal growth and regeneration. Moreover, three unknown proteins with novel sequences were found to be up-regulated by spinal cord injury. Further characterization of these molecules may help us come closer to understanding the mechanisms that underlie the inability of the adult CNS to regenerate. |
| 语种 | 英语 |
| WOS记录号 | WOS:000235207700013 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59177]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, SJ |
| 作者单位 | 1.Inst Basic Med Sci, Dept Neurobiol, Beijing 100850, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Beijing 100080, Peoples R China 3.Chinese Natl Ctr Biomed Anal, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Chem, Beijing Mass Spectrometry Ctr, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, QX,Wu, Z,Guo, YJ,et al. Proteome analysis of up-regulated proteins in the rat spinal cord induced by transection injury[J]. PROTEOMICS,2006,6(2):505-518. |
| APA | Ding, QX.,Wu, Z.,Guo, YJ.,Zhao, CJ.,Jia, YF.,...&Liu, SJ.(2006).Proteome analysis of up-regulated proteins in the rat spinal cord induced by transection injury.PROTEOMICS,6(2),505-518. |
| MLA | Ding, QX,et al."Proteome analysis of up-regulated proteins in the rat spinal cord induced by transection injury".PROTEOMICS 6.2(2006):505-518. |
入库方式： OAI收割
来源：化学研究所
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