Purification and determination of stachyose in Chinese artichoke (Stachys Sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection
文献类型：期刊论文
| 作者 | Yin, Junfa; Yang, Gengliang; Wang, Sumin; Chen, Yi |
| 刊名 | TALANTA
 |
| 出版日期 | 2006-08-15 |
| 卷号 | 70期号:1页码:208-212 |
| 关键词 | Stachyose Chinese Artichoke (Stachys Sieboldii Miq.) Evaporative Light Scattering Detection (Elsd) Carbohydrates |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2006.03.027 |
| 英文摘要 | A high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was applied for simultaneous determination of stachyose, sucrose and raffinose in Chinese artichoke (Stachys Sieboldii Miq.). Carbohydrates were separated on a Bondapak NH2 column using a ternary solvent mixture of methanol-acetonitrile-water (55:25:20, v/v/v) as mobile phase. Two different crafts (method I and method 2) for extraction and purification of stachyose in Chinese artichoke tubers were detailed and evaluated. Method I can meet the purpose of quantification and method 2 is appropriate for the purpose of purification. The content of stachyose in dry tubers was determined to be 236.0 mg/g (from method 1) and the purity of the extracted stachyose flour was calculated to be 87.34% (from method 2), respectively. The analytical method fulfills all the standard requirements of linearity, accuracy and precision. Therefore, it is suitable for purification and routine quantification of stachyose in Chinese artichoke. (c) 2006 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000239709400037 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59393]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Gengliang |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Junfa,Yang, Gengliang,Wang, Sumin,et al. Purification and determination of stachyose in Chinese artichoke (Stachys Sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection[J]. TALANTA,2006,70(1):208-212. |
| APA | Yin, Junfa,Yang, Gengliang,Wang, Sumin,&Chen, Yi.(2006).Purification and determination of stachyose in Chinese artichoke (Stachys Sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection.TALANTA,70(1),208-212. |
| MLA | Yin, Junfa,et al."Purification and determination of stachyose in Chinese artichoke (Stachys Sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection".TALANTA 70.1(2006):208-212. |
入库方式： OAI收割
来源：化学研究所
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