Labeling analysis of neuropeptides with 7-methoxycoumarin-3-carboxylic acid N-succinimidyl ester
文献类型：期刊论文
| 作者 | Jun, Li; Liu Xiang-Jun; Rui, Zhao; Xiong Shao-Xiang; Ma Hui-Min; Qiang, Lu; Li, Xu |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2006-12-10 |
| 卷号 | 27期号:12页码:2297-2299 |
| 关键词 | Neuropeptides Labeling Analysis 7-methoxycoumarin-3-carboxylic Acid N-succimidyl Ester |
| ISSN号 | 0251-0790 |
| 英文摘要 | Labeling analysis of three neuropeptides (Met-enkephalin, Leu-enkephalin, and neurotensin) with 7-methoxycoumarin-3-carboxylic acid N-succinimidyl ester (MCSE) was investigated. The experimental conditions for labeling reaction and HPLC analysis of the neuropeptide derivatives were optimized. It was found that the labeling reatction could be achieved at 37 degrees C for one hour in H3BO3-NaCl-Na2B4O7 buffer (pH = 8.3) with a labeling ratio of about 80%. The derivative mixture from the three peptides can be separated in a 40 min linear gradient from 60% to 20% of solvent A (100 mmol/L sodium acetate, pH = 5.0) and from 40% to 80% of solvent B[60% (volume fraction) acetonitrile in water]. The resulting labeled products were also confirmed by MALDI-TOF-MS analysis. Compared with the native neuropeptides, the resulting labeled products have a longer absorption and fluorescence wavelengths, which is beneficial to the effective elimination of background fluorescence interference from biological matrix. |
| 语种 | 英语 |
| WOS记录号 | WOS:000242962700014 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59447]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma Hui-Min |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 2.Beijing Inst Microchem, Beijing 100091, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jun, Li,Liu Xiang-Jun,Rui, Zhao,et al. Labeling analysis of neuropeptides with 7-methoxycoumarin-3-carboxylic acid N-succinimidyl ester[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2006,27(12):2297-2299. |
| APA | Jun, Li.,Liu Xiang-Jun.,Rui, Zhao.,Xiong Shao-Xiang.,Ma Hui-Min.,...&Li, Xu.(2006).Labeling analysis of neuropeptides with 7-methoxycoumarin-3-carboxylic acid N-succinimidyl ester.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,27(12),2297-2299. |
| MLA | Jun, Li,et al."Labeling analysis of neuropeptides with 7-methoxycoumarin-3-carboxylic acid N-succinimidyl ester".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 27.12(2006):2297-2299. |
入库方式： OAI收割
来源：化学研究所
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