Radiolabeling of filamentous phage peptide library with Tc-99m and its biodistribution in normal mice
文献类型：期刊论文
| 作者 | Sun Li-Yan; Chu Tai-Wei; Wang Xiang-Yun |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 33期号:12页码:1200-1206 |
| 关键词 | Filamentous Phage Display Library Biodistribution Labeling Tc-99m |
| ISSN号 | 1000-3282 |
| 英文摘要 | Numerous peptides that bind to a given target have been selected by phage display technology. However, some peptides isolated to date do not bind with high affinity to tumor or organ sites, even peptides were selected in vivo. Therefore, the biodistribution of Tc-99m-labeled filamentous phage peptide library via MAG3 (mercaptoacetyltriglycine) were investigated to gain a better understanding of phage circulation in vivo. The experimental results showed that the liver and spleen were the organs of the greatest accumulation, while heart, muscle, pancreas and brain retained less radioactivity. In opposite to other tissues and organs, the radioactivity in stomach, intestine and bone gradually went up with time. The clearance of Tc-99m-labeled phage in blood was very fast from 5 min to 30 min and then slowed down. When phage in vivo circulated at enough long period of time, some phage particles could extravasate in some organs or tissues and internalized there. In conclusion, the circulation time of phage in vivo should be experimentally determined beforehand according to the targeted organs and the specific location of target peptides in order to panning a peptide with high specificity and affinity to that target. |
| 语种 | 英语 |
| WOS记录号 | WOS:000243097200008 |
| 出版者 | CHINESE ACAD SCIENCES, INST BIOPHYSICS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59481]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chu Tai-Wei |
| 作者单位 | Peking Univ, Dept Appl Chem, Coll Chem & Mol Engn, BNLMS, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun Li-Yan,Chu Tai-Wei,Wang Xiang-Yun. Radiolabeling of filamentous phage peptide library with Tc-99m and its biodistribution in normal mice[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2006,33(12):1200-1206. |
| APA | Sun Li-Yan,Chu Tai-Wei,&Wang Xiang-Yun.(2006).Radiolabeling of filamentous phage peptide library with Tc-99m and its biodistribution in normal mice.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,33(12),1200-1206. |
| MLA | Sun Li-Yan,et al."Radiolabeling of filamentous phage peptide library with Tc-99m and its biodistribution in normal mice".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 33.12(2006):1200-1206. |
入库方式： OAI收割
来源：化学研究所
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