Characterization of a nitrogenase CrFe protein from a mutant UW3 of Azotobacter vinelandii grown on a Cr-containing medium
文献类型：期刊论文
| 作者 | Zhang Zhigang; Zhao Ying; Zhang Chunxi; Bian Shaomin; Zhou Huina; Wang Huangping; Yin Hong; Huang Jufu |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 51期号:14页码:1729-1735 |
| ISSN号 | 1001-6538 |
| 关键词 | Azotobacter Vinelandii Mutant Uw3 Crfe Protein Metalloclusters Circular Dichroism (Cd) Electron Paramagnetic Resonance (Epr) |
| DOI | 10.1007/s11434-006-2047-7 |
| 英文摘要 | Through the anoxic chromatography on the columns of DEAE-52, Q-Sepharose and Sephacryl S-200, a CrFe protein preparation was obtained from UW3, a mutant of Azotobacter vinelandii, which grew well on the Cr-containing medium. Compared with MoFe protein (OP Av1) from wild-type strain OP, similar to 50% protein in the preparation had similar subunits composition and had immune reaction with antibody of OP Av1. The preparation had similar to 40% of C2H2-, H+- and N-2 (expressed by the difference in H+-reduction activity between under Ar and under N-2) -reduction activity of OP Av1 and similar electron pairs to those of OP Av1. And metal analysis showed that the preparation contained Fe, Cr and Mo. The circular dichroism (CD) spectrum in the preparation at similar to 450 nm was similar to that of OP Av1, while the relative intensities of three EPR signals appearing at the same g values (g approximate to 4.3, 3.7 and 2.0) were different. The EPR-based calculated results showed that (1) the ratios of Mo to Cr and of Fe to (Cr+Mo) of the CrFe protein preparation were 0.41 and 15, respectively, indicating that in the preparation, a ratio of Cr-containing protein to MoFe protein was about 2.5; (2) the ratios of activity to Fe and to Cr of the preparation were close to the ratios of activity to Fe and to Mo of OP Av1, respectively; (3) the ratios of the EPR signal intensities at the above three g values to Cr of CrFe protein were about similar to 83%, 0% and similar to 40% of those to Mo of OP Av1, respectively. The results indicate further that CrFe protein might be a new nitrogenase component I protein with a similar function and structure including metallocluster to those of MoFe protein instead of simple replacement of Mo by Mn in the cofactor. |
| 语种 | 英语 |
| 出版者 | SCIENCE CHINA PRESS |
| WOS记录号 | WOS:000240391900012 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59629]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Huang Jufu |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.NW Univ Xian, Coll Life Sci, Xian 710069, Peoples R China 3.Chinese Acad Sci, Inst Chem, Lab Photochem, Beijing 100080, Peoples R China 4.Fujian Normal Univ, Bioengn Coll, Fuzhou 350007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Zhigang,Zhao Ying,Zhang Chunxi,et al. Characterization of a nitrogenase CrFe protein from a mutant UW3 of Azotobacter vinelandii grown on a Cr-containing medium[J]. CHINESE SCIENCE BULLETIN,2006,51(14):1729-1735. |
| APA | Zhang Zhigang.,Zhao Ying.,Zhang Chunxi.,Bian Shaomin.,Zhou Huina.,...&Huang Jufu.(2006).Characterization of a nitrogenase CrFe protein from a mutant UW3 of Azotobacter vinelandii grown on a Cr-containing medium.CHINESE SCIENCE BULLETIN,51(14),1729-1735. |
| MLA | Zhang Zhigang,et al."Characterization of a nitrogenase CrFe protein from a mutant UW3 of Azotobacter vinelandii grown on a Cr-containing medium".CHINESE SCIENCE BULLETIN 51.14(2006):1729-1735. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。