Determination of H2O2-dependent generation of singlet oxygen from human saliva with a novel chemiluminescence probe
文献类型：期刊论文
| 作者 | Sun, SN; Li, XH; Zhang, GX; Ma, HM; Zhang, DQ; Bao, ZJ |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
 |
| 出版日期 | 2006-03-01 |
| 卷号 | 1760期号:3页码:440-444 |
| 关键词 | Chemiluminescence Singlet Oxygen Generation Human Saliva 4 2-methyl-6-phenyl-3 4 '(5 ')-bis[2-(9-anthryloxy)Ethylthio]Tetrathiafulvalene 7-dihydroimidazo[1 2-a]Pyrazin-3-one |
| ISSN号 | 0304-4165 |
| 英文摘要 | Singlet oxygen (O-1(2)) has been shown to play an important role in salivary defense system, but its generation process and level from human saliva remain uncertain due to the lack of a reliable detection method. We have previously reported 4,4'(5)-bis[2-(9-anthryloxy)ethylthioI tetrathiafulvalene (BAET) as a novel chemiluminescence probe for O-1(2). In this work, the probe is successfully used to characterize H2O2-dependent generation of O-1(2) from saliva in real time. However, the yield of O-1(2) is found to be very low, for example, being about 0.13 nmol from 200 mu L saliva in the presence of 1 mM of hydrogen peroxide over a 5-s reaction period. The result is also compared with that obtained with another O-1(2) probe 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (CLA), demonstrating that, besides O-1(2) the other reactive oxygen species such as hydroxyl radical may also be involved in the reaction of saliva with H2O2. Furthermore, the present study shows that the selectivity of BAET for O-1(2) is much higher than that of CLA and thus BAET is highly suited for the detection of O-1(2) in the presence of other reactive oxygen species in biological systems. (C) 2006 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000236494000018 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59715]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, HM |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, SN,Li, XH,Zhang, GX,et al. Determination of H2O2-dependent generation of singlet oxygen from human saliva with a novel chemiluminescence probe[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2006,1760(3):440-444. |
| APA | Sun, SN,Li, XH,Zhang, GX,Ma, HM,Zhang, DQ,&Bao, ZJ.(2006).Determination of H2O2-dependent generation of singlet oxygen from human saliva with a novel chemiluminescence probe.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1760(3),440-444. |
| MLA | Sun, SN,et al."Determination of H2O2-dependent generation of singlet oxygen from human saliva with a novel chemiluminescence probe".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1760.3(2006):440-444. |
入库方式： OAI收割
来源：化学研究所
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