Direct determination of reduced glutathione in biological fluids by Ce(IV)-quinine chemiluminescence
文献类型：期刊论文
| 作者 | Wang, Shujuan; Ma, Huimin; Li, Jun; Chen, Xinqi; Bao, Zhijuan; Sun, Shuna |
| 刊名 | TALANTA
 |
| 出版日期 | 2006-10-15 |
| 卷号 | 70期号:3页码:518-521 |
| 关键词 | Reduced Glutathione Rat Brain Dialysate Rabbit Whole Blood Chemiluminescence Cerium(Iv)-quinine System |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2005.12.052 |
| 英文摘要 | A simple, sensitive and selective chemiluminescence (CL) method is proposed for the determination of reduced glutathione (GSH) in biological fluids. This method is based on the fact that the weak CL of GSH oxidized with cerium(IV) can be greatly enhanced by quinine. The optimum conditions for the CL emission were investigated. The calibration curve for GSH was linear over the concentration range of 4.0 x 10(-9)-4.0 x 10(-5) M with a detection limit of 5 x 10(-10) M (S/N = 3). The R.S.D. was found to be 4.0% by 11 replicate determinations of 1.0 x 10(-7) M GSH. It was also found that GSH and cysteine, the two often-coexisting thiol compounds in biological samples, exhibited a different CL sensitivity in the Ce(IV)-quinine system (the sensitivity of GSH was higher than that of cysteine). This leads to the successful use of the proposed method for the direct and selective determination of GSH in rabbit whole blood and rat brain microdialysate in the presence of cysteine. Moreover, compared to the classical 5,5'-dithiobis(2-nitrobenzoic acid) method, the present one has the advantages of simplicity and high sensitivity. (c) 2006 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000241063600006 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59745]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, Huimin |
| 作者单位 | Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shujuan,Ma, Huimin,Li, Jun,et al. Direct determination of reduced glutathione in biological fluids by Ce(IV)-quinine chemiluminescence[J]. TALANTA,2006,70(3):518-521. |
| APA | Wang, Shujuan,Ma, Huimin,Li, Jun,Chen, Xinqi,Bao, Zhijuan,&Sun, Shuna.(2006).Direct determination of reduced glutathione in biological fluids by Ce(IV)-quinine chemiluminescence.TALANTA,70(3),518-521. |
| MLA | Wang, Shujuan,et al."Direct determination of reduced glutathione in biological fluids by Ce(IV)-quinine chemiluminescence".TALANTA 70.3(2006):518-521. |
入库方式： OAI收割
来源：化学研究所
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