Preparation of bioconjugates of CdTe nanocrystals for cancer marker detection
文献类型：期刊论文
| 作者 | Hu, FQ; Ran, YL; Zhou, ZA; Gao, MY |
| 刊名 | NANOTECHNOLOGY
 |
| 出版日期 | 2006-06-28 |
| 卷号 | 17期号:12页码:2972-2977 |
| ISSN号 | 0957-4484 |
| DOI | 10.1088/0957-4484/17/12/025 |
| 英文摘要 | Highly fluorescent CdTe quantum dots (Q-dots) stabilized by 3-mercaptopropionic acid (MPA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting a cancer marker, carcinoembryonic antigen (CEA), expressed on human colon carcinoma cell line LS 180. Nonspecific adsorptions of CdTe Q-dots on carcinoma cells were observed and effectively eliminated by replacing MPA with a thiolated PEG ( poly( ethylene glycol), Mn = 750) synthesized according to literature. It was unexpectedly found out that the PEG- coated CdTe Q-dots exhibited very strong and specific affinity to anti-CEA monoclonal antibody rch 24 ( rch 24 mAb). The resultant CdTe -(rch 24 mAb) conjugates were successfully used in detections of CEA expressed on the surface of cell line LS 180. Further experiments demonstrated that the fluorescent CdTe Q-dots exhibited much better photostability and a brighter fluorescence than FITC, which consequently led to a higher efficiency in the cancer marker detection. |
| 语种 | 英语 |
| WOS记录号 | WOS:000238256300025 |
| 出版者 | IOP PUBLISHING LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59791]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ran, YL |
| 作者单位 | 1.Chinese Acad Med Sci, Inst Canc, Dept Cell & Mol Biol, Beijing 100021, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid Interface Sci & Chem Thermodynam, Beijing 100080, Peoples R China 3.Peking Union Med Coll, Beijing 100021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, FQ,Ran, YL,Zhou, ZA,et al. Preparation of bioconjugates of CdTe nanocrystals for cancer marker detection[J]. NANOTECHNOLOGY,2006,17(12):2972-2977. |
| APA | Hu, FQ,Ran, YL,Zhou, ZA,&Gao, MY.(2006).Preparation of bioconjugates of CdTe nanocrystals for cancer marker detection.NANOTECHNOLOGY,17(12),2972-2977. |
| MLA | Hu, FQ,et al."Preparation of bioconjugates of CdTe nanocrystals for cancer marker detection".NANOTECHNOLOGY 17.12(2006):2972-2977. |
入库方式： OAI收割
来源：化学研究所
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