An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase
文献类型：期刊论文
| 作者 | Li Huaqing; Guo Zonghui; Liu Chunxiu; Jiang Liying; Cui Dafu; Liu Wenyong; Huang Yong; Cai Xinxia |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2006-09-01 |
| 卷号 | 34页码:S1-S4 |
| ISSN号 | 0253-3820 |
| 英文摘要 | A two-electrode configuration has been fabricated by depositing gold film on a polyethylene terephthalate (PET) substrate. The integrated reference electrode was obtained by screen-printing commercial silver and silver chloride paste on the gold electrode. The working electrode was modified by drop-coating a redox polymer formed of poly (4-vinylpyrodine) complexed with osmium (2, 2-bipyrridine)(2). The bienzyme, horseradish peroxidase (HRP and glutamate oxidase (GLOx), were immobilized in the redox matrix by the (poly(ethylene glycol) diglycidyl ether(PEGDGE) crosslinker. The optimized biosensor were characterized by a sensitivity of 37.5 mA(mol/L)(-1) cm(-2), a detection limit of 1.0 x 10(-6) mol/L, a response time of less than 16 s, and a correlation coefficient during a concentration ranged from 1.0 x 10(-6) mol/L to 1.0 x 10(-4) mol/L is 0.9998 (n = 9). The biosensor exhibits excellent reproducibility and stability, retaining more than 90% of its original activity over a period of one month. The simple fabrication of the biosensor can be used to determinate L-glutamate rapidly in food industry and clinical diagnose. |
| 语种 | 英语 |
| WOS记录号 | WOS:000244539600001 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59865]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Cai Xinxia |
| 作者单位 | 1.Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Inst Chem, Joint Lab Polymer Sci & Mat, State Key Lab Polymer Phys & Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Huaqing,Guo Zonghui,Liu Chunxiu,et al. An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34:S1-S4. |
| APA | Li Huaqing.,Guo Zonghui.,Liu Chunxiu.,Jiang Liying.,Cui Dafu.,...&Cai Xinxia.(2006).An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34,S1-S4. |
| MLA | Li Huaqing,et al."An L-glutamate amperometric biosensor based on redox polymer wired horseradish peroxidase and glutamate oxidase".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34(2006):S1-S4. |
入库方式： OAI收割
来源：化学研究所
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