Influence of generation 2-5 of PAMAM dendrimer on the inhibition of Tat peptide/TAR RNA binding in HIV-1 transcription
文献类型：期刊论文
| 作者 | Wang, Wei; Guo, Zhenpeng; Chen, Yi; Liu, Tao; Jiang, Long |
| 刊名 | CHEMICAL BIOLOGY & DRUG DESIGN
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 68期号:6页码:314-318 |
| 关键词 | Capillary Electrophoresis Human Immunodeficiency Virus Type 1 Polyamidoamine Dendrimer Quartz Crystal Microbalance Tar Rna Tat |
| ISSN号 | 1747-0277 |
| DOI | 10.1111/j.1747-0285.2006.00454.x |
| 英文摘要 | The special binding of Tat protein to TAR RNA leads to the transcription of HIV-1 virus. In this study, the influence of 2-5 generation of PAMAM dendrimers on the inhibition of Tat protein/TAR RNA binding has been investigated. The absorption of PAMAM dendrimers on TAR RNA, fixed on a gold substrate through an avidin-biotion connection, was carried out by using a quartz crystal microbalance. Experimental result shows a Langmuir-type isotherm could be used to describe this kind of binding, implying a specific and monolayer adsorption existed. The combination coefficient (K-D(-1))(s) can be calculated according to Langmuir Equation, having the order of G3 > G4 > G5 > Tat > G2, indicating that PAMAM G3, G4 and G5 having the possibility to be the inhibitors of HIV-1 transcription. The migration time (T-migra) of capillary electrophoretic technique has the same sequence as (K-D(-1))(s). These two parameters could be used as simple and quantitative criteria for the selection of possible drugs from numerous candidates for HIV therapy in vitro. |
| 语种 | 英语 |
| WOS记录号 | WOS:000242835300004 |
| 出版者 | BLACKWELL PUBLISHING |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59913]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jiang, Long |
| 作者单位 | Chinese Acad Sci, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wei,Guo, Zhenpeng,Chen, Yi,et al. Influence of generation 2-5 of PAMAM dendrimer on the inhibition of Tat peptide/TAR RNA binding in HIV-1 transcription[J]. CHEMICAL BIOLOGY & DRUG DESIGN,2006,68(6):314-318. |
| APA | Wang, Wei,Guo, Zhenpeng,Chen, Yi,Liu, Tao,&Jiang, Long.(2006).Influence of generation 2-5 of PAMAM dendrimer on the inhibition of Tat peptide/TAR RNA binding in HIV-1 transcription.CHEMICAL BIOLOGY & DRUG DESIGN,68(6),314-318. |
| MLA | Wang, Wei,et al."Influence of generation 2-5 of PAMAM dendrimer on the inhibition of Tat peptide/TAR RNA binding in HIV-1 transcription".CHEMICAL BIOLOGY & DRUG DESIGN 68.6(2006):314-318. |
入库方式： OAI收割
来源：化学研究所
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