Label-free reading of microarray-based proteins with high throughput surface plasmon resonance imaging
文献类型：期刊论文
| 作者 | Huang, Haowen; Chen, Yi |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2006-12-15 |
| 卷号 | 22期号:5页码:644-648 |
| 关键词 | Label-free Protein Array Surface Plasmon Resonance Imaging Denaturation |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2006.01.025 |
| 英文摘要 | A simple method is presented discriminating proteins at a gold surface by using an emerging technology, surface plasmon resonance (SPR) imaging. As a high throughput method, the protein array of bovine serum albumin (BSA), poly-L-lysine (PL), casein and lactate dehydrogenase (LDG) was fabricated and SPR imaging enables detection from different kinds of proteins immobilized on the sensor surface. These proteins can be discriminated directly by various reflected intensity or changing the incident angular position of light. Denaturation of these immobilized proteins on SPR sensor by interacting with denaturant 6 M GdnHCl solution was also performed and obvious changes in reflected intensity were occurred after denaturation. The observation of denaturation of these proteins further supported the fact that different proteins could be discriminated on protein array before denaturation. On the other hand, the procedure of denaturation provided useful information that any change of molecular structure with the progress of denaturation would result in change of SPR signal. Excellent reproducibility with a chip-to-chip for label-free discriminating various proteins was achieved. (c) 2006 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000242230800011 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/59927]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Huang, Haowen |
| 作者单位 | 1.Hunan Univ Sci & Technol, Sch Chem & Chem Engn, Xiangtan 411201, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Haowen,Chen, Yi. Label-free reading of microarray-based proteins with high throughput surface plasmon resonance imaging[J]. BIOSENSORS & BIOELECTRONICS,2006,22(5):644-648. |
| APA | Huang, Haowen,&Chen, Yi.(2006).Label-free reading of microarray-based proteins with high throughput surface plasmon resonance imaging.BIOSENSORS & BIOELECTRONICS,22(5),644-648. |
| MLA | Huang, Haowen,et al."Label-free reading of microarray-based proteins with high throughput surface plasmon resonance imaging".BIOSENSORS & BIOELECTRONICS 22.5(2006):644-648. |
入库方式： OAI收割
来源：化学研究所
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