New method for HPLC separation and fluorescence detection of malonaldehyde in normal human plasma
文献类型：期刊论文
| 作者 | Mao, JJ; Zhang, HW; Luo, J; Li, L; Zhao, R; Zhang, RN; Liu, GQ |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2006-02-17 |
| 卷号 | 832期号:1页码:103-108 |
| 关键词 | Malonaldehyde 9-fluorenylmethoxycarbonyl Hydrazine Plasma Fluorescence Detection |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2005.12.041 |
| 英文摘要 | A new method for the detection of free and total malonaldehyde (MDA) in human plasma samples based on the derivatization of MDA with 9-fluorenylmethoxycarbonyl hydrazine (FMOC-hydrazine) in an acidic medium was developed. Derivatization was achieved after 4h at 50 degrees C. The derivatized samples were analyzed by HPLC using a reversed-phase C-18 column with fluorescence detection (Ex = 270 nm, Em = 310 nm). The benefit of this direct injection of deproteinized plasma is to avoid the use of an internal standard. The detection limit was 0.1 pmol (4.0 nmol/L). The recovery of MDA spiked in different human plasma samples was 95.3% (n = 25; R.S.D. 5.1%) for the hydrolysation procedure. The total and free MDA in plasma of 15 healthy male volunteers are 426 +/- 29.8 nmol/L and 153 9.6 nmol/L, respectively. (c) 2006 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000235769100016 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60073]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, GQ |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, JJ,Zhang, HW,Luo, J,et al. New method for HPLC separation and fluorescence detection of malonaldehyde in normal human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,832(1):103-108. |
| APA | Mao, JJ.,Zhang, HW.,Luo, J.,Li, L.,Zhao, R.,...&Liu, GQ.(2006).New method for HPLC separation and fluorescence detection of malonaldehyde in normal human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,832(1),103-108. |
| MLA | Mao, JJ,et al."New method for HPLC separation and fluorescence detection of malonaldehyde in normal human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 832.1(2006):103-108. |
入库方式： OAI收割
来源：化学研究所
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