Molecularly imprinted solid-phase extraction for rapid screening of mycophenolic acid in human plasma
文献类型：期刊论文
| 作者 | Yin, Junfa; Wang, Sumin; Yang, Guanqun; Yang, Gengliang; Chen, Yi |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2006-11-21 |
| 卷号 | 844期号:1页码:142-147 |
| 关键词 | Mycophenolic Acid Molecularly Imprinted Polymer Molecularly Imprinted Solid-phase Extraction |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2006.07.009 |
| 英文摘要 | A molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography (MISPE-HPLC) method was developed for rapid screening of mycophenolic acid (MPA) in human plasma. MPA imprinted polymers (MPA-MIP) were synthesized and then tested for their performance both in organic and in aqueous solution. MPA was selectively trapped and preconcentrated on the MPA-MIP sorbent using different loading and washing conditions. The good selectivity of MPA-MIP enabled further clean-up of the interfering components in human plasma. For the proposed MISPE-HPLC method, the linearity between responses (peak area) and concentration was found over the range of 1-100 mu g/ml with a linear regression coefficient (R-2) of 0.9989. The limit of detection (LOD) and theoretical limit of quantification (LOQ) for MPA in plasma were 0.10 and 0.32 mu g/ml, respectively. The precisions were 7.3, 3.5 and 4.7% RSD for intra-day assay and 9.2, 4.1 and 5.5% RSD for inter-day reproducibility, respectively, at three concentration levels of MPA in spiked plasma (1, 10 and 100 mu g/ml). Both recoveries for the extraction (more than 74%) and for the analytical method (more than 87%) were acceptable for screening MPA in plasma samples. Twelve-hour pharmacokinetic profile of MPA for a renal transplant recipient receiving chronic oral dosing of 500 mg mycophenolate mofetil (MMF) was investigated. Results indicated that this method could be applied for therapeutic drug monitoring of mycophenolic acid in patient plasma. (c) 2006 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000242328300019 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60133]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Gengliang |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Junfa,Wang, Sumin,Yang, Guanqun,et al. Molecularly imprinted solid-phase extraction for rapid screening of mycophenolic acid in human plasma[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2006,844(1):142-147. |
| APA | Yin, Junfa,Wang, Sumin,Yang, Guanqun,Yang, Gengliang,&Chen, Yi.(2006).Molecularly imprinted solid-phase extraction for rapid screening of mycophenolic acid in human plasma.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,844(1),142-147. |
| MLA | Yin, Junfa,et al."Molecularly imprinted solid-phase extraction for rapid screening of mycophenolic acid in human plasma".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 844.1(2006):142-147. |
入库方式： OAI收割
来源：化学研究所
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