Online acid barrage stacking anti-salt injection for capillary electrophoresis of 9-fluorenylmethylchloroformate-derivatized amino acids in high ionic strength solutions by UV detection
文献类型：期刊论文
| 作者 | Han, Yanli; Zuo, Min; Qi, Li; Liu, Kun; Mao, Lanqun; Chen, Yi |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 27期号:21页码:4240-4248 |
| 关键词 | Acid Barrage Stacking Anti-salt Injection Ce 9-fluorenylmethyl Chloroformate-amino Acids Physiological Samples |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200600149 |
| 英文摘要 | An acid barrage stacking (ABS) method has been shown to be feasible for online antisalt injection in CE of 9-fluorenylmethyl chloroformate (FMOC)-labeled amino acids (AAs) detected by common UV absorption. The operation was performed on normal polar CE by sucking in an extra plug of acid following a sample zone, serving as a selective acid barrage to block the backward migration of weak anionic analytes due to a sudden mobility reduction via acid-base reaction which does not affect strong co-ions such as Cl- to penetrate the barrage freely. By CE-UV of FMOC-AAs in various NaCl solutions, the effectiveness of ABS was firmly validated, able to stand up to 500 mM NaCl and to stack analytes by 10(3)-fold calculated from the UV detection limits, that is 0.01 mu M for ABS and 10 mu M for non-stacking injection. The method was also validated by determining trace Glu and Asp in real samples of rat brain microdialysate, rat serum and human saliva. The intraday RSDs were 0.33-4.9% for migration time and 1.8-9.6% for peak area. The recoveries measured by spiking technique were 82-115% for Glu and 86-116% for Asp. Working equations were obtained by plotting peak height vs. concentration at 0.1-50 mu M, with correlation coefficients of > 0.999. The contents of Glu and Asp were thus found at 0.26-0.83 mu M and 0.24-0.64 mu M respectively, in rat brain microdialyste; 37-40 mu M and 8.4-10 mu M, respectively, in rat serum; and 3.5-5.8 mu M and 1.0-4.1 mu M, respectively in human saliva. They were consistent with the data from other methods. |
| 语种 | 英语 |
| WOS记录号 | WOS:000242222500015 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60177]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Yi |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yanli,Zuo, Min,Qi, Li,et al. Online acid barrage stacking anti-salt injection for capillary electrophoresis of 9-fluorenylmethylchloroformate-derivatized amino acids in high ionic strength solutions by UV detection[J]. ELECTROPHORESIS,2006,27(21):4240-4248. |
| APA | Han, Yanli,Zuo, Min,Qi, Li,Liu, Kun,Mao, Lanqun,&Chen, Yi.(2006).Online acid barrage stacking anti-salt injection for capillary electrophoresis of 9-fluorenylmethylchloroformate-derivatized amino acids in high ionic strength solutions by UV detection.ELECTROPHORESIS,27(21),4240-4248. |
| MLA | Han, Yanli,et al."Online acid barrage stacking anti-salt injection for capillary electrophoresis of 9-fluorenylmethylchloroformate-derivatized amino acids in high ionic strength solutions by UV detection".ELECTROPHORESIS 27.21(2006):4240-4248. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。