A fluorescence ratiometric protein assay using light-harvesting conjugated polymers
文献类型：期刊论文
| 作者 | An, Lingling; Tang, Yanli; Wang, Shu; Li, Yuliang; Zhu, Daoben |
| 刊名 | MACROMOLECULAR RAPID COMMUNICATIONS
 |
| 出版日期 | 2006-07-05 |
| 卷号 | 27期号:13页码:993-997 |
| 关键词 | Conjugated Polymers Fluorescent Ratiometric Technique Protein Assay |
| ISSN号 | 1022-1336 |
| DOI | 10.1002/marc.200600214 |
| 英文摘要 | A highly selective protein assay was created which combines the fluorescent ratiometric technique based on FRET with the light-harvesting properties of conjugated polymers. the cationic poly[9,9-bis(6'-N,N,N-trimethylammonium)-hexyl-fluorene phenylene]-bromide (PFP-NMe3+) and the negatively charged biotinylated fluorescein probe (Fl-B) were used to detect the target protein streptavidin optically. the strong, electrostatic interactions between PFP-NMe3+ and fluorescein result in efficient FRET from PFP-NMe3+ to fluorescein. In the presence of streptavidin, however, the biotin moiety of Fl-B specifically associates with streptavidin and the fluorescein molecule is burned deeply in the adjacent vacant binding sites. this separates the fluorescein spatially from the PFP-NMe3+ moiety, resulting in inefficient FRET from PFP-NMe3+ to fluorescein. Although a nonspecific protein, such as BSA, shows nonspecific interactions with PFP-NMe3+, it does not affect the fluorescent ratio value of PFP-NMe3+ to fluorescein. Hence, the charged neutral complex of two oppositely charged conjugated polymers can eliminate the nonspecific interactions, and thus optimize their application in protein assays. |
| 语种 | 英语 |
| WOS记录号 | WOS:000239316200001 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60277]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, Shu |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Lingling,Tang, Yanli,Wang, Shu,et al. A fluorescence ratiometric protein assay using light-harvesting conjugated polymers[J]. MACROMOLECULAR RAPID COMMUNICATIONS,2006,27(13):993-997. |
| APA | An, Lingling,Tang, Yanli,Wang, Shu,Li, Yuliang,&Zhu, Daoben.(2006).A fluorescence ratiometric protein assay using light-harvesting conjugated polymers.MACROMOLECULAR RAPID COMMUNICATIONS,27(13),993-997. |
| MLA | An, Lingling,et al."A fluorescence ratiometric protein assay using light-harvesting conjugated polymers".MACROMOLECULAR RAPID COMMUNICATIONS 27.13(2006):993-997. |
入库方式： OAI收割
来源：化学研究所
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