Immobilization of glucose oxidase onto gold nanoparticles with enhanced thermostability
文献类型：期刊论文
| 作者 | Li, Dongxiang; He, Qiang; Cui, Yue; Duan, Li; Li, Junbai |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2007-04-06 |
| 卷号 | 355期号:2页码:488-493 |
| 关键词 | Immobilization Glucose Oxidase Gold Nanoparticles Bioconjugate Enzyme Activity |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2007.01.183 |
| 英文摘要 | immobilized proteins and enzymes were widely investigated in medical field as well as in food and environmental fields. In this paper, glucose oxidase (GOD) monolayer was covalently immobilized on the surface of gold nanoparticles (AuNPs) to fabricate bioconjugate complex. The citrate-stabilized AuNPs were first functionalized by a carboxyl-terminated alkanethiol and the terminal carboxyl groups were subsequently bonded with side-chain amino groups of protein surface through EDC/NHS coupling reaction. The enzyme activity assays of the obtained bioconjugates display an enhanced thermostability and similar pH-dependence behavior in contrast with that of free enzyme. Such GOD/AuNPs bioconjugates can be considered as a catalytic nanodevice to construct nanoreactor based on glucose oxidation reaction for biotechnological purpose. (c) 2007 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000244774700032 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60293]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Junbai |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, BNLMS, Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China 2.Max Planck Inst Colloids & Interfaces, D-14476 Golm, Germany |
| 推荐引用方式 GB/T 7714 | Li, Dongxiang,He, Qiang,Cui, Yue,et al. Immobilization of glucose oxidase onto gold nanoparticles with enhanced thermostability[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,355(2):488-493. |
| APA | Li, Dongxiang,He, Qiang,Cui, Yue,Duan, Li,&Li, Junbai.(2007).Immobilization of glucose oxidase onto gold nanoparticles with enhanced thermostability.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,355(2),488-493. |
| MLA | Li, Dongxiang,et al."Immobilization of glucose oxidase onto gold nanoparticles with enhanced thermostability".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 355.2(2007):488-493. |
入库方式： OAI收割
来源：化学研究所
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