Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE
文献类型：期刊论文
| 作者 | Qi, Li; Liu, Meirong; Guo, Zhenpeng; Xie, Mingyi; Qiu, Changgui; Chen, Yi |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2007-11-01 |
| 卷号 | 28期号:22页码:4150-4155 |
| 关键词 | Amino Acid Enantioseparation Ligand-exchange Ce Rice-wine Product Zinc Complex |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200700281 |
| 英文摘要 | The aim of this work was to assay seasoning D- or L-aromatic amino acids (AAs) in rice-brewed suspensions, Laozao in Chinese, by chiral ligand-exchange CE with UV detection and Zn(II) complex as a chiral selecting system. Resolution and peak retention were found to be parallel to the basicity of the AA chiral ligands, and basic L-Arg was known to work the best at pH 8.20 compared with L-Lys and other AA ligands. Baseline separation Of DL-aromatic AAs and partially separation of some FMOC-labeled nonaromatic AAs have been achieved using a running buffer of 5 mM ammonium acetate, 100 mM boric acid, 3 mM ZnSO4, and 6 MM L-Arg at pH 8.20. The aromatic amino acids in four brands of Laozao were measured in a range of 0.25-20 mu g/mL for Typ, 1.00-120 mu g/mL for Phe, and 2.50-200 mu g/ml, for Tyr, with linear regression coefficient all over 0.999. The LOD (S/N = 3) was 0.15 mu g/mL for Typ, 0.50 mu g/mL for Phe, and 1.25 mu g/mL for Tyr. The recovery of the method determined by spiking with the supernates of Laozao as background was 94.0-112.9%. The RSDs of migration time and peak area measured from six injections of tyrosine were 0.2 and 2.7%, respectively, for run-to-run, and 1.6 and 3.2%, respectively for day-to-day. Interestingly, there were only L-Trp, D-Tyr, and L-Tyr found in the assayed four brands of Laozao. They may serye as an index to recognize the brand of Laozao. |
| 语种 | 英语 |
| WOS记录号 | WOS:000251363800016 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60375]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Yi |
| 作者单位 | Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Li,Liu, Meirong,Guo, Zhenpeng,et al. Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE[J]. ELECTROPHORESIS,2007,28(22):4150-4155. |
| APA | Qi, Li,Liu, Meirong,Guo, Zhenpeng,Xie, Mingyi,Qiu, Changgui,&Chen, Yi.(2007).Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE.ELECTROPHORESIS,28(22),4150-4155. |
| MLA | Qi, Li,et al."Assay of aromatic amino acid enantiomers in rice-brewed suspensions by chiral ligand-exchange CE".ELECTROPHORESIS 28.22(2007):4150-4155. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。