中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Single molecule fluorescence imaging of thrombin aptamer folding

文献类型:期刊论文

作者Ma Xin-Yong; Wang Jun; Chen Bo; Fang Xiao-Hong
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
出版日期2007-10-01
卷号28期号:10页码:1852-1856
关键词Single Molecule Fluorescence Electron Transfer G-quartet Thrombin Aptamer Folding
ISSN号0251-0790
英文摘要The folding of the thrombin DNA aptamer was studied with single molecule fluorescence imaging. The aptamer was singly labeled with TMR and immobilized on the glass surface. In the presence of K+, the folding of the aptamer resulted in the formation of G-quartets, thus brought TMR close to the guanosines. This led to the fluorescence decrease of TMR through the electron transfer between the dye and guanosines. From the fluorescence intensity of individual TMR, the distribution of aptamer molecules between the folded and random coil conformations was monitored at different concentrations of K+. The results show a new approach for the investigation of aptamer conformational change by electron transfer induced fluorescence quenching, which is complementary to the commonly used fluorescence resonance energy transfer in single molecule study.
语种英语
WOS记录号WOS:000250324400012
出版者HIGHER EDUCATION PRESS
源URL[http://ir.iccas.ac.cn/handle/121111/60409]  
专题中国科学院化学研究所
通讯作者Fang Xiao-Hong
作者单位Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式
GB/T 7714
Ma Xin-Yong,Wang Jun,Chen Bo,et al. Single molecule fluorescence imaging of thrombin aptamer folding[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(10):1852-1856.
APA Ma Xin-Yong,Wang Jun,Chen Bo,&Fang Xiao-Hong.(2007).Single molecule fluorescence imaging of thrombin aptamer folding.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,28(10),1852-1856.
MLA Ma Xin-Yong,et al."Single molecule fluorescence imaging of thrombin aptamer folding".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 28.10(2007):1852-1856.

入库方式: OAI收割

来源:化学研究所

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