Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance
文献类型：期刊论文
| 作者 | Deng, Wei; Cao, Aoneng; Lai, Luhua |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2007-10-26 |
| 卷号 | 362期号:3页码:689-694 |
| 关键词 | Amyloid Fibril Formation Amyloid Fibril Structure Transthyretin (105-115) Fret Parallel P-sheet Electron Microscope |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2007.08.059 |
| 英文摘要 | Transthyretin (TTR) is an amyloidogenic protein involved in many mental diseases. The peptide derived from TTR (105-115) has been widely studied as a model peptide for understanding the mechanism of amyloid fibril formation. However, the detailed arrangement of this peptide in amyloid fibril is still unclear. We have studied the amyloid fibril formation process of TTR (105-115) by introducing a pair of FRET probes into the peptide with a dansyl group at the N-terminal and a tryptophan residue at the C-terminal. Our experiment demonstrated that the strands of TTR (105-115) in the same beta-sheet may be parallel and the mating sheets may be anti-parallel to each other in the amyloid fibril. The kinetics followed by FRET and EM indicated for a possible intermediate state and the distance between sheets became shorter when the intermediate amyloid fibril turns into a more matured form. (c) 2007 Elsevier Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000249602700025 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60423]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Cao, Aoneng |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Wei,Cao, Aoneng,Lai, Luhua. Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,362(3):689-694. |
| APA | Deng, Wei,Cao, Aoneng,&Lai, Luhua.(2007).Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,362(3),689-694. |
| MLA | Deng, Wei,et al."Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 362.3(2007):689-694. |
入库方式： OAI收割
来源：化学研究所
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