A sensitive and homogeneous SNP detection using cationic conjugated polymers
文献类型：期刊论文
| 作者 | Duan, Xinrui; Li, Zhengping; He, Fang; Wang, Shu |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2007-04-11 |
| 卷号 | 129期号:14页码:4154-+ |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/ja070452c |
| 英文摘要 | Single nucleotide polymorphisms (SNPs) comprise the most abundant source of genetic variation in the human genome. A SNP and allele frequency analysis method was established using an optically amplifying water-soluble cationic conjugated polymer and highly selective single base extension reaction in a simple, convenient, homogenous, and sensitive manner. The higher FRET efficiency between PFP and dGTP-Fl is correlated to the incorporation of dGTP-Fl into the probe by one base extension reaction when the target/probe pair is complementary at the polymorphic site. The mutant-type target DNA can be detected in the presence of the wild-type target in which the concentration is much higher than that of mutant-type target. As low as 2% allele frequency can be accurately determined by this new assay method. |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000245723800023 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60667]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Zhengping |
| 作者单位 | 1.Hebei Univ, Coll Chem & Environm Sci, Baoding, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Xinrui,Li, Zhengping,He, Fang,et al. A sensitive and homogeneous SNP detection using cationic conjugated polymers[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2007,129(14):4154-+. |
| APA | Duan, Xinrui,Li, Zhengping,He, Fang,&Wang, Shu.(2007).A sensitive and homogeneous SNP detection using cationic conjugated polymers.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,129(14),4154-+. |
| MLA | Duan, Xinrui,et al."A sensitive and homogeneous SNP detection using cationic conjugated polymers".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 129.14(2007):4154-+. |
入库方式： OAI收割
来源：化学研究所
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