Production and characterization of monoclonal antibody against recombinant human erythropoietin
文献类型：期刊论文
| 作者 | Mi, Jie-Bo; Yan, Jin; Ding, Xia-Jie; Guo, Zhen-Quan; Zhao, Mei-Ping; Chang, Wen-Bao |
| 刊名 | BIOMEDICAL AND ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 20期号:3页码:184-188 |
| 关键词 | Recombinant Human Erythropoietin Monoclonal Antibody Igm Elisa |
| ISSN号 | 0895-3988 |
| 英文摘要 | Objective To produce specific monoclonal antibody (mAb) against recombinant human erythropoietin (rHuEPO) for development of highly efficient methods for erythropoietin detection in biological fluids. Methods rHuEPO was covalently coupled with bovine serum albumin (BSA) and the conjugate was used to immunize mice to produce specific mAb against rHuEPO based on hybridoma technology. The obtained F-3-mAb was characterized by enzyme-linked immunosorbent assay (ELISA), SDS-PAGE and Western blot. Results The isotype of F-3-mAb was found to be IgM with an affinity constant of 2.1x10(8) L/mol. The competitive ELISA using the obtained IgM showed a broader linear range and lower detection limit compared with previous work. Conclusions The modification of rHuEPO was proved to be successful in generating required specific mAb with high avidity to rHuEPO. |
| 语种 | 英语 |
| WOS记录号 | WOS:000247576100002 |
| 出版者 | CHINESE ACAD PREVENTIVE MEDICINE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60789]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Mei-Ping |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mi, Jie-Bo,Yan, Jin,Ding, Xia-Jie,et al. Production and characterization of monoclonal antibody against recombinant human erythropoietin[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2007,20(3):184-188. |
| APA | Mi, Jie-Bo,Yan, Jin,Ding, Xia-Jie,Guo, Zhen-Quan,Zhao, Mei-Ping,&Chang, Wen-Bao.(2007).Production and characterization of monoclonal antibody against recombinant human erythropoietin.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,20(3),184-188. |
| MLA | Mi, Jie-Bo,et al."Production and characterization of monoclonal antibody against recombinant human erythropoietin".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 20.3(2007):184-188. |
入库方式： OAI收割
来源：化学研究所
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