Laccase-catalyzed oxidation and intramolecular cyclization of dopamine: A new method for selective determination of dopamine with laccase/carbon nanotube-based electrochemical biosensors
文献类型：期刊论文
| 作者 | Xiang, Ling; Lin, Yuqing; Yu, Ping; Su, Lei; Mao, Lanqun |
| 刊名 | ELECTROCHIMICA ACTA
 |
| 出版日期 | 2007-03-10 |
| 卷号 | 52期号:12页码:4144-4152 |
| 关键词 | Electrochemical Biosensors Dopamine Laccase Carbon Nanotubes |
| ISSN号 | 0013-4686 |
| DOI | 10.1016/j.electacta.2006.11.040 |
| 英文摘要 | This study demonstrates a new electrochemical method for the selective determination of dopamine (DA) with the coexistence of ascorbic acid (AA) and 3,4-dihydroxyphenylacetic acid (DOPAC) with laccase/multi-walled carbon nanotube (MWNT)-based biosensors prepared by cross-linking laccase into MWNT layer confined onto glassy carbon electrodes. The method described here is essentially based on the chemical reaction properties of DA including oxidation, intramolecular cyclization and disproportionation reactions to finally give 5,6-dihydroxyindoline quinone and on the uses of the two-electron and two-proton reduction of the formed 5,6-dihydroxyindoline quinone to constitute a method for the selective determination of DA at a negative potential that is totally separated from those for the redox processes of AA and DOPAC. Instead of the ECE reactions of DA with the first oxidation of DA being driven electrochemically, laccase is used here as the biocatalyst to drive the first oxidation of DA into its quinone form and thus initialize the sequential reactions of DA finally into 5,6-dihydroxyindoline quinone. In addition, laccase also catalyzes the oxidation of AA and DOPAC into electroinactive species with the concomitant reduction of O-2. As a consequence, a combinational exploitation of the chemical properties inherent in DA and the multifunctional catalytic properties of laccase as well as the excellent electrochemical properties of carbon nanotubes substantially enables the prepared laccase/MYTNT-based biosensors to be well competent for the selective determination of DA with the coexistence of physiological levels of AA and DOPAC. This demonstration offers a new method for the selective determination of DA, which could be potentially employed for the determination of DA in biological systems. (c) 2006 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000245365900030 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/60845]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Mao, Lanqun |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Analyt Chem Life Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Ling,Lin, Yuqing,Yu, Ping,et al. Laccase-catalyzed oxidation and intramolecular cyclization of dopamine: A new method for selective determination of dopamine with laccase/carbon nanotube-based electrochemical biosensors[J]. ELECTROCHIMICA ACTA,2007,52(12):4144-4152. |
| APA | Xiang, Ling,Lin, Yuqing,Yu, Ping,Su, Lei,&Mao, Lanqun.(2007).Laccase-catalyzed oxidation and intramolecular cyclization of dopamine: A new method for selective determination of dopamine with laccase/carbon nanotube-based electrochemical biosensors.ELECTROCHIMICA ACTA,52(12),4144-4152. |
| MLA | Xiang, Ling,et al."Laccase-catalyzed oxidation and intramolecular cyclization of dopamine: A new method for selective determination of dopamine with laccase/carbon nanotube-based electrochemical biosensors".ELECTROCHIMICA ACTA 52.12(2007):4144-4152. |
入库方式： OAI收割
来源：化学研究所
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