Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor
文献类型：期刊论文
| 作者 | Jia, Luo; YanYan, Huang; ShaoXiang, Xiong; GuoQuan, Liu; Rui, Zhao |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 52期号:10页码:1311-1319 |
| 关键词 | Antisense Peptide Degeneracy High-performance Affinity Chromatography Quartz Crystal Microbalance Biosensor Human Interferon-beta |
| ISSN号 | 1001-6538 |
| 英文摘要 | The specific interaction between sense and antisense peptides was studied by high-performance affinity chromatography (HPAC) and quartz crystal microbalance (QCM) biosensor. Fragment 1-14 of human interferon-beta (hIFN-beta) was chosen as sense peptide and its three antisense peptides (AS-IFN 1, AS-IFN 2, and AS-IFN 3) were designed according to the degeneracy of genetic codes. The affinity column was prepared with sense peptide as ligand and the affinity chromatographic behavior was evaluated. Glu-substituted antisense peptide (AS-IFN 3) showed the strongest binding to immobilized sense peptide at pH 7.5. A quartz crystal microbalance-flow injection analysis (QCM-FIA) system was introduced to investigate the recognition process in real-time. The equilibrium dissociation constants between sense peptide and AS-IFN 1, AS-IFN 2 and AS-IFN 3 measured 2.08 x 10(-4), 1.31 x 10(-4) and 2.22 x 10(-5) mol/L, respectively. The mechanism study indicated that the specific recognition between sense peptide and AS-IFN 3 was due to sequence-dependent and multi-modal affinity interaction. |
| 语种 | 英语 |
| WOS记录号 | WOS:000247546100004 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/61115]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Rui, Zhao |
| 作者单位 | Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Luo,YanYan, Huang,ShaoXiang, Xiong,et al. Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor[J]. CHINESE SCIENCE BULLETIN,2007,52(10):1311-1319. |
| APA | Jia, Luo,YanYan, Huang,ShaoXiang, Xiong,GuoQuan, Liu,&Rui, Zhao.(2007).Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor.CHINESE SCIENCE BULLETIN,52(10),1311-1319. |
| MLA | Jia, Luo,et al."Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor".CHINESE SCIENCE BULLETIN 52.10(2007):1311-1319. |
入库方式： OAI收割
来源：化学研究所
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