Analysis of phospholipids by NACE with on-line ESI-MS
文献类型：期刊论文
| 作者 | Gao, Fei; Zhang, Zhengxiang; Fu, Xiaofang; Li, Wei; Wang, Tao; Liu, Huwei |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 28期号:9页码:1418-1425 |
| 关键词 | Ms Nace Phospholipids |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.200600533 |
| 英文摘要 | A hyphenated method of nonaqueous capillary electrophoresis coupled to electrospray ionization mass spectrometry (NACE-ESI-MS) is described for the simultaneous analysis of phospholipids. The best results were obtained with a mixed solution of methanol/ACN (40:60 v/v) containing 20 mM ammonium acetate and 0.5% acetic acid, under the applied voltage of 30 kV and capillary temperature of 25 degrees C. ESI-MS measurements were performed in the negative mode with methanol/ACN (40:60 v/v) containing 50 mM ammonium acetate as sheath liquid at a flow rate of 2 mu L/min. Different phospholipid classes have been successfully separated within 16 min, and the molecular species of every single class have been identified by using MS2 or MS3, which generates characteristic fragments through CID. The developed method has been applied to analyze the phospholipids extracted from rat peritoneal surface and the molecular species of phospholipid classes are presented. |
| 语种 | 英语 |
| WOS记录号 | WOS:000246557400016 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/61117]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Huwei |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Inst Analyt Chem, Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China 2.Peking Univ, Hosp 3, Div Nephrol, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Fei,Zhang, Zhengxiang,Fu, Xiaofang,et al. Analysis of phospholipids by NACE with on-line ESI-MS[J]. ELECTROPHORESIS,2007,28(9):1418-1425. |
| APA | Gao, Fei,Zhang, Zhengxiang,Fu, Xiaofang,Li, Wei,Wang, Tao,&Liu, Huwei.(2007).Analysis of phospholipids by NACE with on-line ESI-MS.ELECTROPHORESIS,28(9),1418-1425. |
| MLA | Gao, Fei,et al."Analysis of phospholipids by NACE with on-line ESI-MS".ELECTROPHORESIS 28.9(2007):1418-1425. |
入库方式： OAI收割
来源：化学研究所
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