Analysis of natural carbohydrate biopolymer-high molecular chitosan and carboxymethyl chitosan by capillary zone electrophoresis
文献类型：期刊论文
| 作者 | Fu, Xiaofang; Huang, Ling; Zhai, Maolin; Li, Wei; Liu, Huwei |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2007-04-05 |
| 卷号 | 68期号:3页码:511-516 |
| 关键词 | Chitosan Caboxymethyl Chitosan Capillary Zone Electrophoresis Separation Analysis |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2006.11.001 |
| 英文摘要 | Chitosan and its derivatives have been widely used in many fields for their various function, there were some difference in bioactivities and applications between them. In this paper, a direct capillary zone electrophoresis (CZE) method without any pre-treatment was developed for separation of chitosan and its derivative, a water soluble chitosan called carboxymethyl chitosan (CM-chitosan), which was proved to be rapid and effective with satisfactory resolution. Optimization of conditions including pH, concentration of the buffer, and length of column were investigated, showing that when employing 50mM sodium phosphate at pH 2.0 and untreated fused silica capillary, high-molecular weight chitosan and CM-chitosan were baseline separated with ultraviolet (UV) detector with satisfactory repeat-ability and excellent linear responses. Therefore, this method could be applied to separate and identify chitosan and CM-chitosan in biological samples and commercial products. (c) 2006 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000245779100015 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/61513]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Huwei |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Inst Appl Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Xiaofang,Huang, Ling,Zhai, Maolin,et al. Analysis of natural carbohydrate biopolymer-high molecular chitosan and carboxymethyl chitosan by capillary zone electrophoresis[J]. CARBOHYDRATE POLYMERS,2007,68(3):511-516. |
| APA | Fu, Xiaofang,Huang, Ling,Zhai, Maolin,Li, Wei,&Liu, Huwei.(2007).Analysis of natural carbohydrate biopolymer-high molecular chitosan and carboxymethyl chitosan by capillary zone electrophoresis.CARBOHYDRATE POLYMERS,68(3),511-516. |
| MLA | Fu, Xiaofang,et al."Analysis of natural carbohydrate biopolymer-high molecular chitosan and carboxymethyl chitosan by capillary zone electrophoresis".CARBOHYDRATE POLYMERS 68.3(2007):511-516. |
入库方式： OAI收割
来源：化学研究所
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