Micellar liquid chromatography with dodecyl dimethyl betaine as an in vitro method for prediction of protein-drug binding
文献类型：期刊论文
| 作者 | Jia, Hongying; Yang, Gengliang; Li, Zhiwei; Xin, Peiyong; Zhao, Yu; Chen, Yi |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2007-03-02 |
| 卷号 | 1143期号:1-2页码:88-97 |
| 关键词 | Micellar Liquid Chromatography Dodecyl Dimethyl Betaine Protein-drug Binding Antidiabetic Drugs Antibacterial |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2006.12.060 |
| 英文摘要 | With the accelerating development of new drugs, there is a high need for rapid and simple screening technologies. In this paper, a new in vitro method, dodecyl dimethyl betaine (BS-12) micellar liquid chromatography (MLC) was presented for prediction of protein-drug binding based on the similar property of BS-12 micelles to protein. The predictive possibility of this method was validated by comparing the retention factors of drugs (antidiabetic and antibacterial drugs) on C-18 modified by different surfactants with those on the protein column. Through the investigation of the concentration and pH effect on the retention of the drugs in BS-12 MLC, quantitative retention-protein binding relationships were established according to the retention factors in 0.2 M BS-12 (pH 7.4) MLC and those on the protein column. According to the relationships established, the protein binding of seven drugs for psychiatric disorders, six potential drugs for antibiotics and four commercial antibiotics were predicted. The results were consistent with those-on the BSA column very well. This indicated, BS-12 MLC was a simple, fast and reproducible method to predict protein-drug binding. (c) 2006 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000244546700011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/61533]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Gengliang |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Hebei Univ, Coll Pharm, Baoding 071002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Hongying,Yang, Gengliang,Li, Zhiwei,et al. Micellar liquid chromatography with dodecyl dimethyl betaine as an in vitro method for prediction of protein-drug binding[J]. JOURNAL OF CHROMATOGRAPHY A,2007,1143(1-2):88-97. |
| APA | Jia, Hongying,Yang, Gengliang,Li, Zhiwei,Xin, Peiyong,Zhao, Yu,&Chen, Yi.(2007).Micellar liquid chromatography with dodecyl dimethyl betaine as an in vitro method for prediction of protein-drug binding.JOURNAL OF CHROMATOGRAPHY A,1143(1-2),88-97. |
| MLA | Jia, Hongying,et al."Micellar liquid chromatography with dodecyl dimethyl betaine as an in vitro method for prediction of protein-drug binding".JOURNAL OF CHROMATOGRAPHY A 1143.1-2(2007):88-97. |
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来源：化学研究所
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