Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography
文献类型：期刊论文
| 作者 | Mengl, Zhe; Wen, Dawei; Sun, Dandan; Gao, Fei; Li, Wei; Liao, Yiping; Liu, Huwei |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2007-07-01 |
| 卷号 | 30期号:10页码:1537-1543 |
| 关键词 | Fast Gas Chromatography Free Long-chain Fatty Acids Human Serum |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.200600344 |
| 英文摘要 | The concentration of long-chain fatty acids (LCFAs) in human serum is closely related to human health. It is therefore important to develop a fast, low-cost, efficient method for their determination. In this study, by using fast temperature programming and micro-bore short capillary columns, a fast gas chromatography method was developed for the direct analysis of non-derivatized LCFAs including n-dodecanoic acid to n-hexacosanic acid (C12:0-C26:0, even numbers only), linoleic acid (C18:2), oleic acid (C18:1), and erucic acid (C22:1) within 4.0 min. Method optimization including extraction and separation conditions is considered, and the analysis of real serum samples is presented. The results show that ten LCFAs were well separated with sufficient resolution, and the detection limit was in the range of 2.8-9.6 mu g/mL. The reproducibility (RSD) for both intra-day and inter-day determination was always less than 15%, and the recoveries for these LCFAs were from 63.1 to 97.0%. |
| 语种 | 英语 |
| WOS记录号 | WOS:000248240000017 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/61581]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Huwei |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn,Minist Educ,Inst, Beijing 100871, Peoples R China 2.Renmin Univ China, Dept Chem, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mengl, Zhe,Wen, Dawei,Sun, Dandan,et al. Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(10):1537-1543. |
| APA | Mengl, Zhe.,Wen, Dawei.,Sun, Dandan.,Gao, Fei.,Li, Wei.,...&Liu, Huwei.(2007).Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography.JOURNAL OF SEPARATION SCIENCE,30(10),1537-1543. |
| MLA | Mengl, Zhe,et al."Rapid determination of C12-C26 non-derivatized fatty acids in human serum by fast gas chromatography".JOURNAL OF SEPARATION SCIENCE 30.10(2007):1537-1543. |
入库方式： OAI收割
来源：化学研究所
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