Gene transfection of hyperbranched PEI grafted by hydrophobic amino acid segment PBLG
文献类型：期刊论文
| 作者 | Tian, Huayu; Xiong, Wei; Wei, Jizheng; Wang, Yu; Chen, Xuesi; Jing, Xiabin; Zhu, Qingyu |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2007-06-01 |
| 卷号 | 28期号:18页码:2899-2907 |
| 关键词 | Hyperbranched Polyethylenimine Gene Carriers Cytotoxicity Transfection |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2007.02.027 |
| 英文摘要 | The complex copolymer of hyperbranched polyethylenimine (PEI) with hydrophobic poly(gamma-benzyl L-glutamate) segment (PBLG) at their chain ends was synthesized. This water-soluble copolymer PEI-PBLG (PP) was characterized for DNA complexation (gel retardation assay, particle size, DNA release and DNase I protection), cell viability and in vitro transfection efficiency. The experiments showed that PP can effectively condense pDNA into particles. Size measurement of the complexes particles indicated that PP/DNA tended to form smaller nanoparticles than those of PEI/DNA, which was caused by the hydrophobic PBLG segments compressing the PP/DNA complex particles in aqueous solution. The representative average size of PP/DNA complex prepared using plasmid DNA (pEGFP-N1, pDNA) was about 96 nm. The condensed pDNA in the PP/pDNA complexes was significantly protected from enzymatic degradation by DNase1. Cytotoxicity studies by MTT colorimetric assays suggested that the PP had much lower toxicity than PEI. The in vitro transfection efficiency of PP/pDNA complexes improved a lot in HeLa cells, Vero cells and 293T cells as compared to that of PEI25K by the expression of Green Fluorescent Protein (GFP) as determined by flow cytometry. Thus, the water-soluble PP copolymer showed considerable potential as carriers for gene delivery. (C) 2007 Elsevier Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000246868000012 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/61651]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Xuesi |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Huayu,Xiong, Wei,Wei, Jizheng,et al. Gene transfection of hyperbranched PEI grafted by hydrophobic amino acid segment PBLG[J]. BIOMATERIALS,2007,28(18):2899-2907. |
| APA | Tian, Huayu.,Xiong, Wei.,Wei, Jizheng.,Wang, Yu.,Chen, Xuesi.,...&Zhu, Qingyu.(2007).Gene transfection of hyperbranched PEI grafted by hydrophobic amino acid segment PBLG.BIOMATERIALS,28(18),2899-2907. |
| MLA | Tian, Huayu,et al."Gene transfection of hyperbranched PEI grafted by hydrophobic amino acid segment PBLG".BIOMATERIALS 28.18(2007):2899-2907. |
入库方式： OAI收割
来源：化学研究所
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