Singlet oxygen generation from the decomposition of alpha-linolenic acid hydroperoxide by cytochrome c and lactoperoxidase
文献类型：期刊论文
| 作者 | Sun, Shuna; Bao, Zhijuan; Ma, Huimin; Zhang, Deqing; Zheng, Xiaoping |
| 刊名 | BIOCHEMISTRY
 |
| 出版日期 | 2007-06-05 |
| 卷号 | 46期号:22页码:6668-6673 |
| ISSN号 | 0006-2960 |
| DOI | 10.1021/bi700178u |
| 英文摘要 | Generation of singlet oxygen is first investigated in the decomposition of polyunsaturated lipid peroxide, alpha-linolenic acid hydroperoxide (LAOOH), by heme-proteins such as cytochrome c and lactoperoxidase. Chemiluminescence and electron spin resonance methods are used to confirm the singlet oxygen generation and quantify its yield. Decomposition products of LAOOH are characterized by HPLC-ESI-MS, which suggests that singlet oxygen is produced via the decomposition of a linear tetraoxide intermediate (Russell's mechanism). Free radicals formed in the decomposition are also identified by the electron spin resonance technique, and the results show that peroxyl, alkyl, and epoxyalkyl radicals are involved. The changes of cytochrome c and lactoperoxidase in the reaction are monitored by UV-visible spectroscopy, revealing the action of a monoelectronic and two-electronic oxidation for cytochrome c and lactoperoxidase, respectively. These results suggest that cytochrome c causes a homolytic reaction of LAOOH, generating alkoxyl radical and then peroxyl radical, which in turn releases singlet oxygen following the Russell mechanism, whereas lactoperoxidase leads to a heterolytic reaction of LAOOH, and the resulting ferryl porphyryl radical of lactoperoxidase abstracts the hydrogen atom from LAOOH to give peroxyl radical and then singlet oxygen. This observation would be important for a better understanding of the damage mechanism of cell membrane or lipoprotein by singlet oxygen and various radicals generated in the peroxidation and decomposition of lipids induced by heme-proteins. |
| 语种 | 英语 |
| WOS记录号 | WOS:000246776800021 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/61679]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, Huimin |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Shuna,Bao, Zhijuan,Ma, Huimin,et al. Singlet oxygen generation from the decomposition of alpha-linolenic acid hydroperoxide by cytochrome c and lactoperoxidase[J]. BIOCHEMISTRY,2007,46(22):6668-6673. |
| APA | Sun, Shuna,Bao, Zhijuan,Ma, Huimin,Zhang, Deqing,&Zheng, Xiaoping.(2007).Singlet oxygen generation from the decomposition of alpha-linolenic acid hydroperoxide by cytochrome c and lactoperoxidase.BIOCHEMISTRY,46(22),6668-6673. |
| MLA | Sun, Shuna,et al."Singlet oxygen generation from the decomposition of alpha-linolenic acid hydroperoxide by cytochrome c and lactoperoxidase".BIOCHEMISTRY 46.22(2007):6668-6673. |
入库方式： OAI收割
来源：化学研究所
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