Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis
文献类型：期刊论文
| 作者 | Zhang, Li-Wei; Ding, Li; Zhang, Xin-Xiang |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 387期号:8页码:2833-2841 |
| 关键词 | Affinity Capillary Electrophoresis Binding Constant Retention Factor Migration Time |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-007-1167-5 |
| 英文摘要 | An estimation method for determination of binding constants of receptors to ligands by affinity capillary electrophoresis was evaluated. On the basis of the theories of pseudostationary phase or so-called dynamic stationary phase, the retention factor (k) was used to represent the interaction between the receptor and ligand. k could be easily deduced from the migration times of the ligand and the receptor. Then, with the linear relationship of k versus the concentration of ligand in the running buffer, the binding constant K (b) was calculated from the slope and intercept. In order to test its feasibility, the calculation method was demonstrated using three model systems: the interactions between vancomycin and N-acetyl-Type=D-Ala-D-Ala, ristocetin and N-acetyl-D-Ala-D-Ala, and carbonic anhydrase B and an arylsulfonamide. Estimated binding constants were compared with those determined by other techniques. The results showed that this estimation method was reliable. This calculation method offers a simple and easy approach to estimating binding constants of ligands to receptors. |
| 语种 | 英语 |
| WOS记录号 | WOS:000245123600027 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/61963]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Xin-Xiang |
| 作者单位 | Peking Univ, Coll Chem, BNLMS, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Li-Wei,Ding, Li,Zhang, Xin-Xiang. Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2007,387(8):2833-2841. |
| APA | Zhang, Li-Wei,Ding, Li,&Zhang, Xin-Xiang.(2007).Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,387(8),2833-2841. |
| MLA | Zhang, Li-Wei,et al."Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 387.8(2007):2833-2841. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。